Identification of sugar moieties in chief cells of the rat fundic gastric glands
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ORIGINAL ARTICLE
Identification of sugar moieties in chief cells of the rat fundic gastric glands Laura Gómez‑Santos1 · Edurne Alonso1 · Olatz Crende1 · Gaskon Ibarretxe1 · Juan Francisco Madrid2 · Francisco José Sáez1 Received: 23 June 2020 / Accepted: 21 September 2020 © Japanese Association of Anatomists 2020
Abstract Many studies have been conducted to determine the composition of the glycoconjugates of the mucus-secreting cells of the fundic glands of the stomach. However, the chief cells of these glands have been largely ignored because they secrete mainly zymogens with a lower glycosylation. The aim of this work was to analyze the glycoconjugates of the gastric chief cells by a battery of 17 different lectins, recognizing Fucose, N-acetylgalactosamine, Galactose, N-acetylneuraminic acid, N-acetylglucosamine and Mannose containing oligosaccharides. Histochemical techniques were performed with several lectins and also combined with two pre-treatments; β-elimination, which removes O-linked oligosaccharides, and incubation with Peptide-N-Gycosidase F, which removes N-linked oligosaccharides. In addition, acid hydrolysis was performed before WGA histochemistry, and incubation with glucose oxidase before Con A labeling. Many lectins did not stain the chief cells. In addition, the presence of O-glycans in the apical cell membrane was demonstrated with the lectins AAL, HPA, MPA/ MPL, PNA, RCA-I, and WGA. Some of these O-glycans were resistant to short-term β-elimination pre-treatments. Mannosebinding lectins stained the basal cytoplasm of the chief cells. The level of glycosylation of the chief cells was lower than that of the mucous cells. The presence of O-glycans in the apical cell membrane is consistent with the presence of mucins such as MUC1 in the apical membrane of chief cells. Moreover, Mannose-binding lectins revealed N-glycosylation in the basal cytoplasm. The knowledge of gastric chief cell glycoconjugates is relevant because of their potential involvement not only in in physiological but also in pathological processes, such as cancer. Keywords Gastric glands · Glycoconjugates · Lectin histochemistry · Mucins · Zymogens
Introduction The study of glycoconjugates has been relatively uncommon for a long time, probably due to their great variety and structural complexity. However, in recent years, the analysis of glycans present in glycoproteins and glycolipids—called glycobiology—has become a rapidly growing field in the life sciences, with a great relevance in many areas of basic * Francisco José Sáez [email protected] 1
Department of Cell Biology and Histology, Faculty of Medicine and Nursing, University of the Basque Country UPV/EHU, Bº Sarriena S/N, 48940 Leioa, Vizcaya, Spain
Department of Cell Biology and Histology, Regional Campus of International Excellence “Campus Mare Nostrum”, IMIB‑Arrixaca, University of Murcia, Espinardo, Murcia, Spain
2
research, biomedicine and biotechnology (Varki and Kornfeld 2017). In addition, glycans have been shown to play a large variety of biologic
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