Immobilization of cell membrane onto a glucose-Zn-based porous coordination polymer and its application to rapid screeni
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ORIGINAL PAPER
Immobilization of cell membrane onto a glucose-Zn-based porous coordination polymer and its application to rapid screening of potentially active compounds from Vaccinium corymbosum L. leaves Yan Li 1 & Yanli Chen 1 & Huixia Zhang 1 & Christopher Wai Kei Lam 2 & Zheng Li 1 & Caiyun Wang 1 & Yunfeng Zhao 3 & Wei Zhang 1 & Zhihong Jiang 1 Received: 23 July 2020 / Accepted: 20 October 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract A novel glucose-Zn-based porous coordination polymer (PCP) was selected as a carrier of cell membranes (CMs) to fabricate CM-coated PCP (CMPCP) for rapid screening of potentially active compounds from natural products. The cell disruption and the amount of maximum CMs adsorbed on PCP were optimized according to the amount of immobilized protein. This new kind of matrix exhibited good reproducibility and stability, and was applied for fishing potentially active compounds from the extracts of Vaccinium corymbosum L. leaves (VCL). Using LC-MS/MS, chlorogenic acid and quercetin were identified as the potentially active compounds through comparison of normal and non-alcoholic fatty liver disease (NAFLD)–modeled CMPCP. Our results suggested that the proposed approach based on CMPCP was environmentally friendly, cost-effective, and convenient in terms of green porous material, stable protein loading capacity, and accessible operation process. The developed method could provide a promising platform for efficient drug discovery from natural product resources. Keywords Glucose-Zn-based porous coordination polymer . Cell membrane . Active compounds screening . Natural products
Introduction Yan Li and Yanli Chen contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04612-0) contains supplementary material, which is available to authorized users. * Yunfeng Zhao [email protected] * Wei Zhang [email protected] * Zhihong Jiang [email protected] 1
State Key Laboratory of Quality Research in Chinese Medicines, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa, Macau 999078, China
2
Faculty of Medicine, Macau University of Science and Technology, Taipa, Macau 999078, China
3
Tianjin Key Laboratory of Advanced Functional Porous Materials, School of Materials Science and Engineering, Tianjin University of Technology, Tianjin 300384, China
Natural products have been considered to be a precious resource of biologically active secondary metabolites, which are also widely used as the main source of drug candidates for the treatment of many diseases [1, 2]. Due to their complex chemical constitution, it is difficult to estimate and identify the active components in natural products, which may be related with their various biological activities [3]. Therefore, the development of reliable approaches to screen biologically active compounds is important for new drug discovery. Ligand fishing has emerged as a convenient and
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