Impaired UTP-induced relaxation in the carotid arteries of spontaneously hypertensive rats
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Impaired UTP-induced relaxation in the carotid arteries of spontaneously hypertensive rats Takayuki Matsumoto 1 Tsuneo Kobayashi 1
&
Mihoka Kojima 1 & Keisuke Takayanagi 1 & Tomoki Katome 1 & Kumiko Taguchi 1 &
Received: 9 March 2020 / Accepted: 10 August 2020 # Springer Nature B.V. 2020
Abstract Uridine 5′-triphosphate (UTP) has an important role as an extracellular signaling molecule that regulates inflammation, angiogenesis, and vascular tone. While chronic hypertension has been shown to promote alterations in arterial vascular tone regulation, carotid artery responses to UTP under hypertensive conditions have remained unclear. The present study investigated carotid artery responses to UTP in spontaneously hypertensive rats (SHR) and control Wistar Kyoto rats (WKY). Accordingly, our results found that although UTP promotes concentration-dependent relaxation in isolated carotid artery segments from both SHR and WKY after pretreatment with phenylephrine, SHR exhibited significantly lower arterial relaxation responses compared with WKY. Moreover, UTP-induced relaxation was substantially reduced by endothelial denudation and by the nitric oxide (NO) synthase inhibitor NG-nitro-L-arginine in both SHR and WKY. The difference in UTP-induced relaxation between both groups was abolished by the selective P2Y2 receptor antagonist AR-C118925XX and the cyclooxygenase (COX) inhibitor indomethacin but not by the thromboxane-prostanoid receptor antagonist SQ29548. Furthermore, we detected the release of PGE2, PGF2α, and PGI2 in the carotid arteries of SHR and WKY, both at baseline and in response to UTP. UTP administration also increased TXA2 levels in WKY but not SHR. Overall, our results suggest that UTP-induced relaxation in carotid arteries is impaired in SHR perhaps due to impaired P2Y2 receptor signaling, reductions in endothelial NO, and increases in the levels of COX-derived vasoconstrictor prostanoids. Keywords Carotid artery . Hypertension . P2Y2 . Relaxation . UTP
Abbreviations ADP Adenosine 5′-diphosphate ANOVA Analysis of variance ATP Adenosine 5′-triphosphate COX Cyclooxygenase EDCF Endothelium-derived contracting factor EDHF Endothelium-derived hyperpolarizing factor
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11302-020-09721-2) contains supplementary material, which is available to authorized users. * Takayuki Matsumoto [email protected] * Tsuneo Kobayashi [email protected] 1
Department of Physiology and Morphology, Institute of Medicinal Chemistry, Hoshi University, Shinagawa-ku, Tokyo 142-8501, Japan
EDRFs GPCR KHS L-NNA NO NOS PE SHR UDP Up4A UTP VSMC WKY
Endothelium-derived relaxing factors G protein-coupled receptor Krebs–Henseleit solution NG-nitro-L-arginine Nitric oxide Nitric oxide synthase Phenylephrine Spontaneously hypertensive rat Uridine 5′-diphosphate Uridine adenosine tetraphosphate Uridine 5′-triphosphate Vascular smooth muscle cell Wistar Kyoto
Introduction Adenosine triphosphate (ATP), adenosine diphosphate (AD
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