Influences of Regulation of miR-126 on Inflammation,Th17/Treg Subpopulation Differentiation, and Lymphocyte Apoptosis th
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ORIGINAL ARTICLE
Influences of Regulation of miR-126 on Inflammation,Th17/Treg Subpopulation Differentiation, and Lymphocyte Apoptosis through Caspase Signaling Pathway in Sepsis Qi Zou ,1 Mei Yang,2 Meiling Yu,3 and Cheng Liu1,4
To observe the inflammatory response, differentiation of Th17/Treg subsets and apoptosis of lymphocytes, by regulating miR-126 in lymphocytes of septic rats. After using cecal ligation and puncture to establish sepsis model, miR126 mimic and miR-126 inhibitor were used to transfect lymphocytes of septic rats in vitro and in vivo. ELISA was used to detect TNF-α, IL-6, IL-17, and IL-10, the differentiation of Th17 and Treg was measured by flow cytometry, and apoptosis of lymphocytes was observed by fluorescence microscope; the changes of caspase signaling pathway were detected by immunofluorescence, PCR, and Western blotting. The result show that the expression of miR-126 increased in sepsis. After overexpression of miR-126, the release of TNF-α, IL-6, and IL-17 decreased; the release of IL-10 increased; T lymphocyte subsets differentiated toward Treg; caspase signaling pathway weakened; and lymphocyte of apoptosis decreased compared with sepsis group. While, after inhibition of miR-126, the release of TNF-α, IL-6, and IL-17 increased; the release of IL-10 decreased; T lymphocyte subsets differentiated toward TH17; caspase signaling pathway enhanced; and lymphocyte of apoptosis increased compared with sepsis group. Taken together, regulation of miR-126 can alter the inflammatory response, differentiation of T lymphocyte subsets, and apoptosis of lymphocytes in septic rats.
Abstract—
KEY WORDS: miR-126; Sepsis; Apoptosis; TH17; Treg.
Qi Zou and Mei Yang contributed equally to this work. 1
Department of Critical Care Medicine, The first Affiliated Hospital of Bengbu Medical College, Anhui 233004 Bengbu, People’s Republic of China 2 Department of Pediatrics, The first Affiliated Hospital of Bengbu Medical College, Anhui 233004 Bengbu, People’s Republic of China 3 Department of Pharmacy, The first Affiliated Hospital of Bengbu Medical College, Anhui 233004 Bengbu, People’s Republic of China 4 To whom correspondence should be addressed at Department of Critical Care Medicine, The first Affiliated Hospital of Bengbu Medical College, Anhui 233004 Bengbu, People’s Republic of China. E-mail: [email protected]
INTRODUCTION Sepsis is systemic inflammatory response induced in infection, which emphasizes not only the existence of infection but also the systemic inflammatory response [1]. This kind of inflammatory response is actually the compensatory anti-inflammatory response syndrome (CARS) [2], which is the interactive state of immune function hyperactivity and immune function inhibition, leading to immune function disorder or even collapse [3]. In these immune responses, inflammatory factors and
0360-3997/20/0000-0001/0 # 2020 Springer Science+Business Media, LLC, part of Springer Nature
Zou, Yang, Yu, and Liu immune cells play a major role. These inflammatory factors include TNF-α, IL-6, I
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