Influences of Xeno-Free Media on Mesenchymal Stem Cell Expansion for Clinical Application
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Online ISSN 2212-5469
REVIEW ARTICLE
Influences of Xeno-Free Media on Mesenchymal Stem Cell Expansion for Clinical Application Hue Thi Hong Bui1 • Liem Thanh Nguyen2,3 • Uyen Thi Trang Than2
Received: 19 August 2020 / Revised: 21 September 2020 / Accepted: 27 September 2020 Ó The Korean Tissue Engineering and Regenerative Medicine Society 2020
Abstract Mesenchymal stem/stromal cells (MSCs) are multipotent somatic stem/progenitor cells that can be isolated from various tissues and have attracted increasing attention from the scientific community. This is due to MSCs showing great potential for incurable disease treatment, and most applications of MSCs involve tissue degeneration and treatment of immune- and inflammation-mediated diseases. Conventional MSC cultures contain fetal bovine serum (FBS), which is a common supplement for cell development but is also a risk factor for exposure to animal-derived pathogens. To avoid the risks resulting from the xenogeneic origin and animal-derived pathogens of FBS, xeno-free media have been developed and commercialized to satisfy MSC expansion demands for human clinical applications. This review summarized and provided an overview of xeno-free media that are currently used for MSC expansion. Additionally, we discussed the influences of different xeno-free media on MSC biology with particular regard to cell morphology, surface marker expression, proliferation, differentiation and immunomodulation. The xeno-free media can be serum-free and xeno-free media or media supplemented with some human-originating substances, such as human serum, human platelet lysates, human umbilical cord serum/plasma, or human plasma-derived supplements for cell culture medium. These media have capacity to maintain a spindle-shaped morphology, the expression of typical surface markers, and the capacity of multipotent differentiation and immunomodulation of MSCs. Xeno-free media showed potential for safe use for human clinical treatment. However, the influences of these xeno-free media on MSCs are various and any xeno-free medium should be examined prior to being used for MSC cultures. Keywords Mesenchymal stem cells Xeno-free media Xeno-free and serum-free media Clinical application
1 Introduction
& Uyen Thi Trang Than [email protected] 1
Vinmec HiTech Center, Vinmec Healthcare System, Hanoi, 458 Minh Khai, Hanoi 10000, Vietnam
2
Vinmec Research Institute of Stem Cell and Gene Technology (VRISG), Vinmec Healthcare system, Hanoi 10000, Vietnam
3
College of Health Sciences, Vin University, Hanoi, Vietnam
Mesenchymal stem/stromal cells (MSCs) were first described by Friedenstein et al. in 1970 and are among the most broadly studied adult multipotent stem cells due to their tremendous potential for disease and regenerative treatment [1–3]. This cell type can be obtained from several tissues, such as bone marrow, adipose tissue, umbilical cord, dental pulp, and peripheral blood [4]. According to the characterization criteria published by the International Society for Cellular Therapy (I
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