Interhomolog Homologous Recombination in Mouse Embryonic Stem Cells
Homologous recombination is a critical mechanism for the repair of DNA double-strand breaks (DSBs). It occurs predominantly between identical sister chromatids and at lower frequency can also occur between homologs. Interhomolog homologous recombination (
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Andrés Aguilera Aura Carreira Editors
Homologous Recombination Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, UK
For further volumes: http://www.springer.com/series/7651
For over 35 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-bystep fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice. These hallmark features were introduced by series editor Dr. John Walker and constitute the key ingredient in each and every volume of the Methods in Molecular Biology series. Tested and trusted, comprehensive and reliable, all protocols from the series are indexed in PubMed.
Homologous Recombination Methods and Protocols
Edited by
Andrés Aguilera Centro Andaluz de Biología Molecular y Medicina Regenerativa, CABIMER, Universidad de Sevilla, Sevilla, Spain
Aura Carreira Institut Curie, PSL Research University, CNRS, UMR3348, F-91405, Orsay, France; University Paris Sud, Paris-Saclay University, CNRS, UMR3348, F-91405, Orsay, France
Editors Andre´s Aguilera Centro Andaluz de Biologı´a Molecular y Medicina Regenerativa, CABIMER Universidad de Sevilla Sevilla, Spain
Aura Carreira Institut Curie, PSL Research University, CNRS UMR3348, F-91405 Orsay, France University Paris Sud, Paris-Saclay University, CNRS UMR3348, F-91405 Orsay, France
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-0716-0643-8 ISBN 978-1-0716-0644-5 (eBook) https://doi.org/10.1007/978-1-0716-0644-5 © Springer Science+Business Media, LLC, part of Springer Nature 2021 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither t
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