Interkingdom interaction between C. albicans and S. salivarius on titanium surfaces
- PDF / 1,132,877 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 13 Downloads / 167 Views
020) 20:349
RESEARCH ARTICLE
Open Access
Interkingdom interaction between C. albicans and S. salivarius on titanium surfaces Loyse Martorano‑Fernandes1 , Nadiny Cezar Rodrigues2 , Maria Heloísa de Souza Borges2 , Yuri Wanderley Cavalcanti3 and Leopoldina de Fátima Dantas de Almeida3*
Abstract Background: In oral candidiasis models, Candida albicans and Streptococcus salivarius sp. biofilms have an antagonis‑ tic relationship. Due to this, S. salivarius have been used experimentally as probiotic. However, the interaction between these microorganisms in the peri-implantitis-like microenvironment remains unknown. This study aimed to evaluate the interaction between C. albicans and S. salivarius biofilms developed on titanium surfaces, under reduced oxygen levels. Methods: Titanium specimens were pre-conditioned with artificial saliva (1 h, 37 °C). Single-species biofilms of C. albicans (ATCC 90028) and co-culture biofilms of C. albicans and S. salivarius (ATCC 7073) was developed for 24 and 72 h on titanium specimens. Subsequently, the effect of these intervals of biofilm formation and the interactions among the cells were evaluated. Biofilms from cultures were collected and analyzed for cell viability (CFU/mL), biofilm biomass, and total protein dosage. Data were analyzed using Mann–Whitney test (α = 5%). In addition, co-culture biofilms were analyzed using fluorescence microscopy. Results: C. albicans growth did not change due to the presence of S. salivarius. Besides, co-culture biofilms showed a significant difference in the number of viable cells between 24 and 72 h of biofilm development (p
Data Loading...
