Introgression and confirmation of everbearing trait in strawberry ( Fragaria x ananassa Duch.)
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RESEARCH ARTICLE
Introgression and confirmation of everbearing trait in strawberry (Fragaria x ananassa Duch.) Shivanti Negi1 • Girish Sharma2 • Rajnish Sharma1
Received: 21 August 2020 / Revised: 21 August 2020 / Accepted: 25 November 2020 Ó Prof. H.S. Srivastava Foundation for Science and Society 2020
Abstract The present investigation was targeted towards a highly desirable everbearing trait in strawberry (Fragaria 9 ananassa Duch.) via marker assisted selection while seeing its worldwide commercial applicability through the extended harvest season. The crosses were made between everbearing and june-bearing cultivars to raise the F1 individuals. Morphological characters (plant, floral, and fruit) were assessed that showed significant differences among the strawberry cultivars. Molecular characterization was carried out between everbearing and non-everbearing cultivars using RAPD and SSR markers. For phenotyping, a chi-square test was performed and revealed that out of all four cross combinations, the best fitted cross found to be in Mendelian segregation ratio (1:1) was ‘Confectura’ 9 ‘Torrey’ with v2-value 1.58. Further, the identified polymorphic markers were assessed across the F1 individuals of cross ‘Confectura’ 9 ‘Torrey’ for its genotyping. It could be revealed that the targeted everbearing trait is governed by a dominant gene(s) in the subjected strawberry genotypes. Further, the identified polymorphic markers would be successfully employed in
Supplementary information The online version of this article (https://doi.org/10.1007/s12298-020-00916-w) contains supplementary material, which is available to authorized users. & Rajnish Sharma [email protected] 1
Department of Biotechnology, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh 173 230, India
2
Department of Fruit Science, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh 173 230, India
DNA fingerprinting of strawberry under various crop improvement programme. Keywords Everbearing trait Fruit Molecular breeding Morphology Polymorphism Strawberry
Introduction The Strawberry (Fragaria 9 ananassa Duch.) is the most economically important, nutritious soft fruit rich in vitamins, antioxidants, carbohydrates and belongs to the family Rosaceae (Rousseau-Gueutin et al. 2009). It is vegetatively propagated outbred species derived from the hybridization of F. chiloensis and F. virginiana. Conventionally, strawberry cultivars are commercially propagated through runners and crowns. The cultivated strawberry is an octoploid (2n = 8x = 56) and its fleshy fruits are not derived from the ovaries but from the receptacle that holds the ovaries. Globally, the area under cultivation recorded was 3,73,435 ha with a total production of 91,18,336 MT (FAO 2017). In India, strawberry is cultivated on around one thousand hctares with a total production of five thousand MT (Anonymous 2019). Two primary types of commercial strawberries are generally grown viz., the everbeari
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