Invited Discussion on: Effects of Harvest Sites on Cryopreserved Adipose-Derived Stem Cells and ASC-Enriched Fat Grafts
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EDITOR’S INVITED COMMENTARY
Invited Discussion on: Effects of Harvest Sites on Cryopreserved Adipose-Derived Stem Cells and ASC-Enriched Fat Grafts Steven R. Cohen1,2
•
Angelo Trivisonno3 • J. Sarah Crowley2
Received: 28 August 2020 / Accepted: 28 August 2020 Ó Springer Science+Business Media, LLC, part of Springer Nature and International Society of Aesthetic Plastic Surgery 2020
Level of Evidence V This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266. Thank you for the opportunity to discuss the article entitled: ‘‘Effects of Harvest Sites on Cryopreserved Adipose-derived Stem Cells and ASC-enriched fat grafts’’ [1]. This study was conducted on 18 healthy volunteers who underwent liposuction. Twenty mL lipoaspirates was obtained from four sites, the upper limb, abdomen, waist, and thighs, by the Coleman technique. Adipose-derived stem cells were ex vivo cultured and cryopreserved for 4 weeks. The biological characteristics of ASCs from four harvest sites were analyzed for MSC surface markers, cell proliferation, migration ability, and their multipotential differentiation. The fat grafts were co-implanted with ASCs from the four harvest sites and injected subcutaneously in mice. The grafts were analyzed 3 months after transplantation. The cryopreserved ASCs from the abdomen and thighs maintained more significant cell proliferation, migration ability, and differentiation potential, compared with cells from the upper limb and waist. Better graft retention of cell-assisted fat grafts with cryopreserved
& Steven R. Cohen [email protected] 1
FACES ? Plastic Surgery, Skin and Laser Center, Rome, Italy
2
Department of Surgery, Division of Plastic Surgery, UC San Diego School of Medicine, 4510 Executive Drive Suite 200, San Diego, CA 92121, USA
3
Private Practice, Plastic Surgery, Rome, Italy
ASC from the abdomen and thighs was noted when compared to those from the waist and upper limb. There are several important points this article addresses. One is that different harvest sites seem to have different cell characteristics in spite of similar cell morphology and surface markers. Fat tissue from thighs was found to have the highest SVF yield and cell viability, followed by the abdomen. The percentages of ASCs (CD31-CD34?CD45-) in SVF from the abdomen and the thighs are significantly higher compared with the upper limb and the waist. Results from CCK-8 assay showed higher viability of cell proliferation in ASCs from thighs compared to other sites. Results of the scratch test showed that the migration ability of ASCs from the abdomen and thighs was significantly higher than that from the upper limb and waist at 6 and 24 h. The authors found that in terms of multilineage differentiation, cryopreserved ASCs from four harvest sites had the potential abilities of adipogenic, osteogenic, and chondrogenic differentiation. However
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