Level of in vitro storage of the European catfish ( Silurus glanis L.) eggs at different temperatures
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Level of in vitro storage of the European catfish (Silurus glanis L.) eggs at different temperatures Merve Tinkir & Devrim Memiş & Yu Cheng & Miaomiao Xin & Marek Rodina & David Gela & Vladimíra Tučková & Otomar Linhart
Received: 29 April 2020 / Accepted: 17 November 2020 # Springer Nature B.V. 2020
Abstract European catfish (Silurus glanis) is a commercially important freshwater fish originating from Eastern Europe. The objective of this study was to examine the short-term storage of its eggs especially in relation to maintaining a low level of malformation in newly hatched fry. The eggs from freshly spawned individuals were stored separately in cell incubators at 17 and 22 °C under aerobic conditions. Changes in fertilization, hatching, and malformation were examined in eggs stored at 1, 3, 5, and 7 h post-stripping. The sperm used for fertilization showed very good motility rates (84–90%) and curvilinear velocity (110–125 μm/ s), and straight-line velocity did not drop below 77 μm/ s. For all females, a temperature of 17 °C was better than 22 °C for egg storage in vitro. Egg fertilization and total hatching decreased rapidly after 7 h storage at 17 °C. The storage time of eggs in vitro to fertilization should
: D. Memiş
M. Tinkir Faculty of Aquatic Sciences, Istanbul University, Ordu Str. No. 08, Laleli, 34134 Istanbul, Turkey Y. Cheng : M. Xin : M. Rodina : D. Gela : V. Tučková : O. Linhart (*) Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceske Budejovice, Zatisi 728/II, 38925 Vodnany, Czech Republic e-mail: [email protected] Y. Cheng : M. Xin Sino-Czech Joint Laboratory for Fish Conservation and Biotechnology, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, China
therefore not exceed 5 h at 17 °C. In all females, there was no difference in the total number of eggs hatching between 1 and 3 h of egg storage at 17 °C. The storage time of eggs did not correlate with the level of malformations of the fry. However, the level of hatching and malformations was clearly affected by the storage temperature of eggs when it was > 17 °C. Analysis showed that the storage time of eggs, temperature of storage, and individual females had a significant influence on fertilization and total hatching rates. Regression analysis confirmed a low correlation of fertilization and hatching rates with storage time of eggs. Keywords Egg . Fertilization . Fish reproduction . Spermatozoa . Silurus glanis
Introduction European catfish (Silurus glanis L., 1758; also called wels catfish or sheath fish) are commercially important freshwater fish originating from Eastern European countries (Linhart et al. 2002). They live in streams and lakes of Central and Eastern Europe and West Asia and are among the 20 largest freshwater fishes worldwide, attaining a total length > 2.7 m and a documented body weight of 130 kg (Bouletreau and Santoul
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