Long non-coding RNA Mirt2 relieves lipopolysaccharide-induced injury in PC12 cells by suppressing miR-429
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ORIGINAL ARTICLE
Long non-coding RNA Mirt2 relieves lipopolysaccharide-induced injury in PC12 cells by suppressing miR-429 Haibo Li 1 & Yu Xu 1 & Guoxiu Wang 2 & Xuerong Chen 1 & Wenqing Liang 1 & Huawei Ni 3 Received: 4 January 2019 / Accepted: 26 June 2019 # University of Navarra 2019
Abstract Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) play important roles in the pathogenesis of spinal cord injury (SCI). This study investigated the effects of lncRNA Mirt2 and miR-429 on lipopolysaccharide (LPS)induced injuries in PC12 cells. Serum samples were collected from 36 patients with SCI and the healthy controls. The expression of lncRNA Mirt2 in serum samples was measured by qRT-PCR. The in vitro model of SCI was established by treating PC12 cells with LPS. The effects of lncRNA Mirt2 and miR-429 on the cell model were evaluated by CCK-8 assay, flow cytometry, western blot, qRT-PCR, and ELISA. Further, the activation of NF-κB and p38MAPK pathways was tested by western blot. LPS induced obvious cell injuries in PC12 cells, as cell viability was reduced, apoptosis rate was increased, caspase-3 and -9 were cleaved, and the release of TNF-α and IL-6 was induced. lncRNA Mirt2 was up-regulated in LPS-stimulated PC12 cells and serum samples derived from SCI patients. Overexpression of lncRNA Mirt2 protected PC12 cells against LPS-induced injuries. Further studies found that lncRNA Mirt2 acted as the molecular sponge of miR-429 and miR-34a-5p. lncRNA Mirt2 did not protect PC12 cells when miR-429 was overexpressed. Moreover, the inhibitory effects of lncRNA Mirt2 on NF-κB and p38MAPK pathways were abolished when miR-429 was overexpressed. lncRNA Mirt2 exerts protective effects in an in vitro model of SCI by down-regulating miR-429. This study shed light on the treatment of SCI by using the lncRNA-miRNA regulation network. Keywords Mirt2 . Spinal cord injury . miR-429 . Inflammation . Apoptosis
Haibo Li and Yu Xu co-first authors Statement Shaoxing People’s Hospital (Shaoxing Hospital of Zhejiang University), Shenzhen University General Hospital, and The Affiliated Hospital of Hangzhou Normal University did not provide our authors the institutional email addresses. So we can only use our personal email address to submit the paper. We confirm that they are not fake email addresses. The authors can be reached at their email addresses. * Huawei Ni [email protected] 1
Department of Orthopaedics, Shaoxing People’s Hospital (Shaoxing Hospital of Zhejiang University), Shaoxing 312000, China
2
Department of Orthopaedics, Shenzhen University General Hospital, Shenzhen 518055, China
3
Department of Orthopaedics, The Affiliated Hospital of Hangzhou Normal University, No.126 Wenzhou Road, Hangzhou 310015, China
Introduction Spinal cord injury (SCI) is a kind of central nervous system (CNS) trauma with high disability rate. According to statistics, the incidence of SCI in USA is approximately 24–77 per million people [3, 5]. Despite that the pathogenesis of SCI has not been known clearly, it is generally believed that o
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