LTBP1 plays a potential bridge between depressive disorder and glioblastoma
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Journal of Translational Medicine Open Access
RESEARCH
LTBP1 plays a potential bridge between depressive disorder and glioblastoma Xiaojun Fu1,2 , Pei Zhang3, Hongwang Song4, Chenxing Wu1, Shengzhen Li3, Shouwei Li1* and Changxiang Yan1*
Abstract Background: Glioblastoma multiforme (GBM) is the most malignant tumor in human brain. Diagnosis and treatment of GBM may lead to psychological disorders such as depressive and anxiety disorders. There was no research focusing on the correlation between depressive/anxiety disorder and the outcome of GBM. Thus, the aim of this study was to investigate the possibility of depressive/anxiety disorder correlated with the outcome of GBM patients, as well as the overlapped mechanism bridge which could link depressive/anxiety disorders and GBM. Methods: Patient Health Questionnaire (PHQ-9) and Generalized Anxiety Disorder (GAD-7) were used to investigate the psychological condition of GBM patients in our department. To further explore the potential mechanism, bioinformatic methods were used to screen out genes that could be indicators of outcome in GBM, followed by gene ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, and protein–protein interaction (PPI) analysis. Further, cellular experiments were conducted to evaluate the proliferation, migration capacity of primary GBM cells from the patients. Results: It was revealed that patients with higher PHQ-9 and GAD-7 scores had significantly worse prognosis than their lower-scored counterparts. Bioinformatic mining revealed that LTBP1 could be a potential genetic mechanism in both depressive/anxiety disorder and GBM. Primary GBM cells with different expression level of LTBP1 should significantly different proliferation and migration capacity. GO, KEGG analysis confirmed that extracellular matrix (ECM) was the most enriched function of LTBP1. PPI network showed the interaction of proteins altered by LTBP1. Hub genes COL1A2, COL5A1 and COL10A1, as well as mesenchymal marker CD44 and Vimentin were statistically higher expressed in LTBP1 high group; while proneural marker E-cadherin was significantly higher expressed in low LTBP1 group. Conclusion: There is closely correlation between depressive/anxiety disorders and GBM. LTBP1 could be a potential bridge linking the two diseases through the regulation of ECM. Keywords: Glioblastoma, Depressive disorder, Anxiety disorder, Bioinformatic, Patient outcome Background Glioblastoma (GBM) is the most common primary WHO grade IV brain tumor in adults [1]. Tens of thousands GBM cases have been confirmed every year all *Correspondence: [email protected]; [email protected] 1 Department of Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Xiangshanyikesong 50#, HaiDian District, Beijing 100093, China Full list of author information is available at the end of the article
over the world [2]. Moreover, GBM is also the most malignant tumor with a median survival time of only 18–24 months, and approximately 13,000 patients in the USA die of GBM each
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