Marker aided introgression of opaque 2 (o2) allele improving lysine and tryptophan in maize ( Zea mays L.)
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Marker aided introgression of opaque 2 (o2) allele improving lysine and tryptophan in maize (Zea mays L.) Bharathi Pukalenthy1 • Dhasarathan Manickam2 • Karthikeyan Adhimoolam2 • Sandesh Goragundi Mahesh1 • Nagalakshmi Ramanathan3 • Sarankumar Chandran4 Vellaikumar Sampathrajan2 • Ravikesavan Rajasekaran 5 • Kavithapushpam Arunachalam6 • Kalaiselvi Senthil7 • Vignesh Muthusamy8 • Firoz Hossain8 • Senthil Natesan4
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Received: 14 February 2020 / Revised: 14 June 2020 / Accepted: 30 July 2020 Ó Prof. H.S. Srivastava Foundation for Science and Society 2020
Abstract Maize is the predominant food source for the world population, but lack of lysine and tryptophan in maize endosperm cannot fulfill the nutritional requirements of humans. Hence, the improvement of lysine and tryptophan content is the ultimate goal of maize biofortification programs. In the present study, the marker-assisted backcross (MABC) breeding strategy was used to enhance the lysine and tryptophan content of the elite maize inbred line UMI1230 by introgressing opaque 2 (o2) gene from the VQL1. During the transfer of the gene into UMI1230, SSR marker umc1066 tightly associated with o2 used for foreground selection. Background recovery was estimated using 168 SSR markers. Phenotype screening for morphological traits was adopted to choose plants parallel to UMI1230. As a result, four BC2F3 improved lines (DBT5-1-14/25-5/25-8/258/25, DBT5-1-14/25-5/25-8/25-7/25, DBT5-1-14/25-5/25-8/
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12298-020-00857-4) contains supplementary material, which is available to authorized users. & Senthil Natesan [email protected] 1
2
Department of Plant Breeding and Genetics, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai, Tamil Nadu, India Department of Biotechnology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai, Tamil Nadu, India
3
Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
4
Department of Plant Molecular Biology and Bioinformatics, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
25-10/25 and DBT5-1-14/25-5/25-8/25-12/25) with o2 were developed. The improved line’s background genome recovery varied between 90.60 and 94.80%. Also, the improved lines had better agronomic performance along with increased lysine (0.311–0.331%) and tryptophan (0.040–0.048%) contents. In summary, the MABC breeding strategy has successfully improved the levels of lysine and tryptophan in UMI1230 without affecting agronomic performance. The improved line’s hold great potential as donors in biofortification programs in maize. Keywords Biofortification Maize Marker-assisted backcrossing opaque 2
Introduction Maize (Zea mays) is not only an important cereal crop of the world but also a primary nutritional source for humans. The maize kernel conta
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