Mechanism of action of Panax notoginoside against lung cancer in mice based on response to CTSB gene
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(2020) 20:367
BMC Complementary Medicine and Therapies
RESEARCH ARTICLE
Open Access
Mechanism of action of Panax notoginoside against lung cancer in mice based on response to CTSB gene Jizhou Zhang1,2, Bin Zhou1, Song Jin1, Zhiyou Huang1, Bidong Ma1, Qiqi Shao3 and Wenzong Zhu4*
Abstract Background: This study aimed to investigate the mechanism of action of Panax notoginoside (PNS) against lung cancer and inhibition of lung cancer cell proliferation by the drug at different concentrations in a mouse model, considering the cathepsin B (CTSB) gene as a target. Methods: The mice were randomly assigned into the following five groups: normal control, tumor-bearing, low-dose Panax notoginoside (TSPN), medium-dose TSPN, and high-dose TSPN. All mice were treated with physiological saline or TSPN at different concentrations for 28 days consecutively by gavage. The tumor size was measured, the tumor growth was observed, and the survival curve was drawn. At different time points, the expression of the CTSB gene was detected using quantitative fluorescent polymerase chain reaction, Western blot analysis, and indirect immunofluorescence. The serum indices, such as carcinoembryonic antigen (CEA), neuron-specific enolase (NSE), and Soluble fragment of cytokeratin 19 (CYFRA21), were detected by enzyme-linked immunosorbent assay. Results: In vivo, PNS could directly inhibit the expression of the CTSB gene in tumors of mice, limit tumor growth, and alter tumor-related indices, such as CEA, NSE, and CYFRA21 levels, in the serum to different extents simultaneously. Conclusion: CTSB gene was closely related to the pathogenesis of lung cancer. PNS could act on the CTSB gene, downregulate the expression of CTSB in lung cancer cells, inhibit the proliferation and invasion of tumors, and prolong the survival period. Keywords: CTSB gene, Inhibitory effect, Lung cancer, Panax notoginoside, Proliferation
Background Kaur [1] indicated an increase in knowledge on the role of phytodaptogens in moderating biological and molecular processes against carcinogenic cells with a prospect of fascinating possibilities. The well-known adaptogens and immunomodulators, such as Rhodiola rosea and Panax notoginseng, have been shown to have significant * Correspondence: [email protected] 4 Internal Medicine of Traditional Chinese Medicine, Wenzhou Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese Medicine University, No. 9 Jiaowei Road, Lucheng District, Wenzhou, China Full list of author information is available at the end of the article
antioxidant and anticarcinogenic properties due to the presence of various biologically active chemical compounds. This strongly supported the view that the beneficial properties of plant adaptogens should be contemplated as an adjuvant because they hold immense potential in the fight against cancer with their capability in orchestrating molecular mechanisms in restoring homeostasis in the body system. Panax notoginoside (PNS) is the main active ingredient in traditional Chinese medicine, Notoginse
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