Methods in Non-Aqueous Enzymology
Extending the range of enzymatic catalysis by using non-aqueous media has now developed into a powerful approach in biochemistry and biotechnology. One peculiar feature which distinguishes it from the conventional enzymology (carried out in aqueous buffer
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Methods in Non-Aqueous Enzymology Edited by Munishwar Nath Gupta
Springer Base! AG
Editor Prof. Dr. Munishwar Nath Gupta Chemistry Department Indian Institute ofTechnology, Delhi Hauz Khas, New Delhi 110016 India Library of Congress Cataloging-in-Publication Data Methods in non-aqueous enzymology / edited by Munishwar Nath Gupta. p. cm. - (Methods and tools in biosciences and medicine) Includes bibliographical references and index. ISBN 978-3-7643-6109-9 ISBN 978-3-0348-8472-3 (eBook) DOI 10.1007/978-3-0348-8472-3 1. Enzymes. 2. Nonaqueous solvents. 3. Enzymology. 4. Lipase. 1. Gupta, Munishwar
Nath. II. Series.
QP601 .M494 2000 572'.7-dc21
00-021710
Deutsche Bibliothek Cataloging-in-Publication Data Methods in Non-Aqueous Enzymology / ed. by: Munishwar Nath Gupta. - Springer Basel AG 2000 (Methods and tools, in biosciences and medicine) ISBN 978-3-7643-6109-9
The publisher and editor can give no guarantee for the information on drug dosage and administration contained in this publication. The respective user must check its accuracy by consulting other sources ofreference in each individual case. The use of registered names, trademarks etc. in this publication, even if not identified as such, does not imply that they are exempt from the relevant protective laws and regulations or free for general use. This work is subject to copyright. AlI rights are reserved, whether the whole or part of the material is concerned, specifically the rights oftranslation, reprinting, re-use ofillustrations, re citation, broadcasting, reproduction on microfilms or in other ways, and storage in data banks. For any kind ofuse permission ofthe copyright owner must be obtained. © 2000 Springer Basel AG
Originally published by Birkhiiuser Verlag, BaseI - Boston - Berlin in 2000
Printed on acid-free paper produced from chlorine-free pulp. TCF ~ Cover Illustration: Enantiomeric exesses ofproduct and substrate vs conversion values (page 19) ISBN 978-3-7643-6109-9
987654321
ISBN 3-7643-5803-3
v
Contents List of Contributors Preface 1. Non-Aqueous Enzymology: Issues and Perspectives Munishwar N. Gupta 2. Importance of Water Activity for Enzyme Catalysis in Non-Aqueous Organic Systems Thorleif Anthonsen and Birte J. Sjursnes
VII IX 1
14
3. Engineering of Enzymes via Immobilization and Post-Immobilization Techniques: Preparation of Enzyme Derivatives with Improved Stability in Organic Media 36 Gloria Fermindez-Lorente, Roberto Fernandez-Lafuente, Pilar Armisen, Pilar Sabuquillo, Cesar Mateo and Jose M. Guisan 4. Immobilization of Lipases for Use in Non-Aqueous Reaction Systems John A. Bosley and Alan D. Peilow
52
5. Applications of Enzymes and Membrane Technology in Fat and Oil Processing Mitsutoshi Nakajima, Jonathan B. Snape and Sunil Kumar Khare
70
6. Strategies for Improving the Lipase-Catalyzed Preparation of Chiral Compounds Uwe T. Bornscheuer
90
7. Peptide Synthesis in Non-Aqueous Media 110 Pere Clapes, Gloria Caminal, Josep Anton Feliu and Josep Lopez-Santin 8. Enzyme Selectivity in Organic Media Gianluca Ottolina
