Microfluidic Cell Volume Biosensor for High Throughput Drug Screening
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Microfluidic Cell Volume Biosensor for High Throughput Drug Screening Daniel A. Ateya1, Frederick Sachs2 and Susan Z. Hua1,2,* 1
Bio-MEMS and Bio-Materials Laboratory, Department of Mechanical and Aerospace Engineering, SUNY-Buffalo, Buffalo, NY 14260, USA 2 Hughes Center for Single Molecule Biophysics, Department of Physiology and Biophysics, SUNY-Buffalo, Buffalo, NY 14214, USA * Corresponding author: S.Z.H., E-mail: [email protected] ABSTRACT The maintenance of cell volume is critical to health. Cell volume change reflects many biological and physiological processes. We have developed a lab-chip to measure cell volume change in real-time with high sensitivity and resolution, and applicable to both adherent and suspended cell populations. The volume change was detected by measuring the impedance of extra-cellular solution within a microfluidic chamber containing the cells. Using microfabrication to make precise chamber dimensions, volume change can be detected in response to an osmotic gradient
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