Micron scale electromagnetic viable tumor cell capture and release
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RESEARCH PAPER
Micron scale electromagnetic viable tumor cell capture and release Yagmur Akin Yildirim1 · Burak Yildirim1 · Tan A. Ince2,3 · Onur Tigli1,4,5 Received: 27 March 2020 / Accepted: 29 July 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract In this study, we report on new developments, improved results and a new fabrication approach to a micro-electromagnetbased microfluidic device capable of capturing and releasing superparamagnetic beads and bead-bound MCF-7 cancer cells on a sensor site with high efficiency based on the challenges identified and reported in our preliminary studies (Gajasinghe et al. 2016). The device is biocompatible and fabricated by utilizing only traditional microfabrication methods. The microfluidic channel is coated with SU-8 to provide uniform and smooth channel surface for easy release of the captured viable cells without any chemical usage. We labelled MCF-7 cells with superparamagnetic beads via Ep-CAM antibody and observed capture rates of the superparamagnetic beads and MCF-7 cells are 99.9 ± 0.2%, and 94.4 ± 7%, and release efficiencies are 97.4 ± 2%, and 95.2 ± 3%, respectively. Furthermore, there was no significant degrade on viability was observed. Reusability of the device was also presented. Overall, our device is highly efficient, fast, low cost, and has potential be used for cell analysis, drug screening, and disease diagnostics. Keywords Cell capture and release · Single cell analysis · Micro-electromagnets · Electromagnetic cell capture · Lab-on-achip · Circulating tumor cells
1 Introduction Circulating tumor cells (CTCs) are cancer cells that emanate from tumor site and circulate around the body (Shen et al. 2013; Shi et al. 2017), causing metastasis, which is a Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10404-020-02378-8) contains supplementary material, which is available to authorized users. * Yagmur Akin Yildirim [email protected] Burak Yildirim [email protected] Onur Tigli [email protected] 1
Electrical and Computer Engineering, University of Miami, Coral Gables, FL, USA
2
Department of Pathology, New York Presbyterian-Brooklyn Methodist Hospital, Brooklyn, NY, USA
3
Weill Cornell Medicine, New York, NY, USA
4
Department of Pathology, Miller School of Medicine, University of Miami, Miami, FL, USA
5
Dr. John T. Macdonald Foundation Biomedical Nanotechnology Institute at University of Miami, Miami, FL, USA
primary cause of death (Kang et al. 2017; Krebs et al. 2010; Wittekind and Neid 2005). Detection and analysis of CTCs are vital, since they could be used for prediction (Mathew et al. 2015) and prognostics (Cheng et al. 2017) of cancer, and their responses to different therapies (Kang et al. 2017). Hence, detection, separation and analysis of the CTCs and other rare tumor cells have become high demand topics in the last two decades, and various devices have been produced for these purposes using different properties of the cells, such as siz
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