microRNA-4270-5p inhibits cancer cell proliferation and metastasis in hepatocellular carcinoma by targeting SATB2
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RESEARCH ARTICLE
microRNA‑4270‑5p inhibits cancer cell proliferation and metastasis in hepatocellular carcinoma by targeting SATB2 Yun Wang1 · Chang‑feng Li2 · Li‑bo Sun3 · Yong‑chao Li3 Received: 21 February 2020 / Accepted: 27 May 2020 © Japan Human Cell Society 2020
Abstract Hepatocellular carcinoma (HCC) remains a lethal cancer type for both males and females. MicroRNAs (miRNAs) contribute to the initiation, development and metastasis of cancer. Although several miRNAs have been identified as drivers or suppressors of HCC, the molecular mechanisms of many miRNAs have not been investigated. Currently, we discovered that miR-4270-5p was a significantly downregulated miRNA in HCC. We revealed that miR-4270-5p overexpression inhibited cell proliferation and invasion of HCC cells. The data manifested that miR-4270-5p directly targeted SATB2, a key regulator of epithelial mesenchymal transition (EMT), in HCC cells and reversed the EMT process. The rescue experiments suggested that SATB2 overexpression reversed the biological function of miR-4270-5p in HCC cells. Clinical data indicated that SATB2 expression was negatively correlated with miR-4270-5p levels in HCC patients. Our findings provided potential targets for prognosis and treatment of patients with HCC. Keywords microRNA-4270-5p · Hepatocellular carcinoma · SATB2
Introduction In 2018, there was an estimated 841,000 newly diagnosed liver cancer cases worldwide, being the sixth leading cause of cancer related death for both sexes [1]. Hepatocellular carcinoma (HCC) is the most commonly diagnosed liver cancer type [2]. It is known that chronic infection with hepatitis B virus (HBV), alcohol intake, smoking are risk factors for HCC [3]. The convention treatment for patients with HCC included surgery removal of tumors and liver transplantation [4]. However, due to the aggressive nature of HCC cells, patients with HCC often develop recurrence and metastasis and the prognosis of these patients remains dismal [5, 6]. It is urgent to investigate the molecular mechanism of HCC to provide novel targets for diagnosis and treatment of patients. * Yong‑chao Li [email protected] 1
Department of Internal Medicine, Liver and Biliary Disease Hospital of Jilin Province, Changchun, Jilin, China
2
Department of Endoscopy Center, The China-Japan Union Hospital of Jilin University Hospital, Changchun, Jilin, China
3
Department of Gastrointestinal Colorectal Surgery, The China-Japan Union Hospital of Jilin University, Xiantai Road No.126, Changchun 130033, Jilin, China
microRNAs (miRNAs) are short, single-stranded nucleotides with no protein coding potential [7]. Via directly binding to complementary sites in the 3′UTR of gene mRNAs, miRNAs facilitate mRNA degradation and inhibition of translation [8]. Transcriptomic studies have revealed that many miRNAs were differentially expressed in HCC compared with normal tissues [9]. Accumulating evidences suggested that miRNAs were involved in HCC progression via targeting key oncogenes and tumor suppressors [10, 11,
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