Microsatellite markers for the endangered orchids Cattleya labiata Lindl. and C. warneri T. Moore (Orchidaceae)
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TECHNICAL NOTE
Microsatellite markers for the endangered orchids Cattleya labiata Lindl. and C. warneri T. Moore (Orchidaceae) Paulo Ricardo M. Almeida • M. Cristina Lo´pez-Roberts Bianca B. Zanotto Vigna • Anete P. Souza • Aristo´teles Go´es-Neto • Ca´ssio van den Berg
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Received: 17 February 2013 / Accepted: 15 March 2013 Ó Springer Science+Business Media Dordrecht 2013
Abstract In this study, we developed a new set of microsatellite markers for endangered Cattleya labiata and Cattleya warneri. Also the first microsatellite loci for Cattleya, these markers can be useful for studies in the conservation genetics of these species. Twenty-one new microsatellite primers pairs were characterized by cloning an enriched library, and two libraries were obtained by cloning inter-simple sequence repeats. Thirteen polymorphic primers were found, and the remaining eight were monomorphic for both species. Our results indicate that these new microsatellite markers have the potential to be used as a tool for studying the genetic diversity of C. labiata and C. warneri and for future studies focusing mating systems and the detection of hybrids. Keywords Cattleya SSR Genetic diversity Conservation genetics
Cattleya Lindl. is a large-flowered orchid genus occurring from Costa Rica to Southern Brazil, Argentina and Bolivia.
P. R. M. Almeida (&) M. C. Lo´pez-Roberts A. Go´es-Neto C. van den Berg Departamento de Cieˆncias Biolo´gicas, Universidade Estadual de Feira de Santana, Av. Transnordestina s.n., Feira de Santana, Bahia 44036-900, Brazil e-mail: [email protected] B. B. Z. Vigna Embrapa Southeast Livestock, CP 339, Sa˜o Carlos, Sa˜o Paulo 13560-970, Brazil A. P. Souza Centro de Biologia Molecular e Engenharia Gene´tica (CBMEG) e Instituto de Biologia, Universidade Estadual de Campinas, CP 6010, Campinas, Sa˜o Paulo 13083-970, Brazil
The genus belongs to the Neotropical subtribe Laeliinae, and recent studies have enlarged its circumscription with the inclusion of many species from Laelia and Sophronitis (Van den Berg 2008; Van den Berg et al. 2009). Cattleya labiata has a broad distribution in northeastern Brazil, and C. warneri occurs 400 km to the south (in a disjunction of approx. 400 km) in southeastern Brazil (Barros et al. 2010). Cattleya labiata and C. warneri are closely related, and the natural populations of both species have been destroyed by deforestation, fires and poaching by professional and amateur collectors. In September 2008, the Brazilian Environment Ministry (MMA) published a new red list of species threatened with extinction (http://www.ibama.gov. br/documentos/lista-de-especies-ameacadas-de-extincao), including both C. labiata and C. warneri and reflecting both their scarcity in the wild and the desirability of the two species. In an attempt to provide tools for the study of population genetics in Cattleya species, we developed a set microsatellite markers for C. labiata and C. warneri and tested their transferability between these species. Microsatellite loci for C. labiata were is
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