miR168 targets Argonaute1A mediated miRNAs regulation pathways in response to potassium deficiency stress in tomato
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RESEARCH ARTICLE
Open Access
miR168 targets Argonaute1A mediated miRNAs regulation pathways in response to potassium deficiency stress in tomato Xin Liu1,2,3, Chunchang Tan1,2,3, Xin Cheng1,2,3, Xiaoming Zhao1, Tianlai Li1,2,3 and Jing Jiang1,2,3*
Abstract Background: Potassium (K+) is an essential ion for most plants, as it is involved in the regulation of growth and development. K+ homeostasis in plant cells has evolved to facilitate plant adaptation to K+-deficiency stress. Argonaute1 (AGO1) is regulated by miR168 to modulate the small RNA regulatory pathway by RNA silencing complex (RISC) in tomatoes. However, the role of miR168-mediated regulation of AGO1 in the context of K+ deficiency stress in tomatoes has not been elucidated yet. Results: SlmiR168 and its target gene SlAGO1A were differentially expressed among low-K+-tolerant JZ34 and lowK+-sensitive JZ18 tomato plants. Transgenic tomato plants constitutively expressing pri-SlmiR168a showed stronger root system growth, better leaves development, and higher K+ contents in roots under K+-deficiency stress than those of the transgenic tomato lines expressing rSlAGO1A (SlmiR168-resistant) and the wild type (WT). Deep sequencing analysis showed that 62 known microRNAs (miRNAs) were up-regulated in 35S:rSlAGO1 compared with WT tomatoes. The same miRNAs were down-regulated in 35S:SlmiR168a compared with WT plants. The integrated analysis found 12 miRNA/mRNA pairs from the 62 miRNAs, including the root growth and cytokinin (CTK)/abscisic acid (ABA) pathways. Conclusions: The regulation mediated by SlmiR168 of SlAGO1A contributes to the plant development under low-K+ stress. Moreover, this regulation mechanism may influence downstream miRNA pathways in response to low-K+ stress through the CTK/ABA and root growth modulation pathways. Keywords: Argonaute1, miR168, microRNA, Potassium deficiency stress, Root, Target genes
Background Macronutrients and micronutrients are the elements required necessarily for growth and development of plants [1]. N (Nitrogen), P (Phosphorus) and K (Potassium) were needed at relatively large amounts for plants. Phosphorus deficiency could reduces the plant growth * Correspondence: [email protected] 1 Horticulture Department, College of Horticulture, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang 110866, P.R. China 2 Key Laboratory of Protected Horticulture of Ministry of Education, No. 120 Dongling Road, Shenhe District, Shenyang 110866, P.R. China Full list of author information is available at the end of the article
and biomass production [2, 3]. It is more important especially in tomato production systems, which require substantial inputs of nitrogen fertilizer [4]. K+ is also a kind of the essential macronutrients that is involved in many physiological processes in plant cells, such as osmoregulation, ion homeostasis, photosynthesis, membrane potential maintenance, cell turgor, and others [5]. These functions rely on a high and relatively stable concentration of K+ in cellular compartments and
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