MiR396 regulatory network and its expression during grain development in wheat
- PDF / 1,258,551 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 7 Downloads / 163 Views
ORIGINAL ARTICLE
MiR396 regulatory network and its expression during grain development in wheat Yi Yu 1 & Fangyao Sun 1 & Ning Chen 2 & Genlou Sun 2
&
Cheng-Yu Wang 1,3 & De-Xiang Wu 1
Received: 17 July 2020 / Accepted: 5 September 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract Wheat contains the largest number of miR396 family with 17 miR396 in Poaceae. MiR396 regulatory network underlying wheat grain development has not comprehensively been explored. Our results showed that precursor miR396 family in Poaceae exhibited not only conservativeness but also diversification especially in wheat. Five haplotypes were detected in Poaceae species, while 4 haplotypes in wheat with Hap-4 (miR396a) and Hap-5 (miR396n) unique to wheat. GO enrichment analysis of target genes showed that the first 20 enrichment functions of miR396a and miR396n are completely different from each other, and also completely different from miR396(b–g), miR396(h–m), and miR396(o–q). Functional annotation on the 18 target genes shared by miR396(b–g), miR396(h–m), and miR396(o–q) found that 11 of the 18 target genes are growth-regulating factor (GRF) genes. Our results indicated that, during the grain filling stage of wheat, miR396 is involved in the development of grains by regulating the expression of GRF genes (GRF1, GRF6, and GRF9). Although the enrichment function of miR396(b–g), miR396(h–m), and miR396(o–q) is the same, the gene functional networks they formed differ greatly. Our results indicated that polyploidization enriches not only the diversity of miR396 family and its target genes but also gene functional networks in wheat. These results laid foundation for further elucidating function of miR396 gene family underlying wheat grain development. Keywords Wheat . miRNA . Target gene . Diversification . Poaceae
Introduction Yi Yu and Fangyao Sun contributed equally to this work. Handling Editor: Peter Nick Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00709-020-01556-3) contains supplementary material, which is available to authorized users. * Genlou Sun [email protected] * Cheng-Yu Wang [email protected] * De-Xiang Wu [email protected] 1
College of Agronomy, Anhui Agricultural University, Hefei 230036, Anhui, China
2
Biology Department, Saint Mary’s University, Halifax, NS B3H 3C3, Canada
3
Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow & Huai River Valley, Ministry of Agriculture, Hefei 230036, China
MicroRNAs (miRNAs) are a class of endogenous singlestranded and non-coding small RNAs with a length of about 22 nucleotide (nt) that exist in eukaryotes. They are determined by endogenous MIR-encoding genes (Pasquinelli and Ruvkun 2002). MiRNAs are located in non-coding regions of genes, have no open reading frame (ORF), and do not encode proteins for regulating the growth and development of organisms (Kim 2005). Instead, they regulate the expression of their target genes at the post-transcriptional levels in three ways:
Data Loading...
