Modern Clinical Molecular Techniques
This timely book covers the need to know clinical practices for all those involved in molecular laboratory science. The field of molecular medicine is evolving at an astounding speed. Propelled by the new insights and technologies, advances are being made
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Peter C. Hu
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Madhuri R. Hegde
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Patrick Alan Lennon
Editors
Modern Clinical Molecular Techniques
Editors Peter C. Hu Molecular Genetic Technology Program School of Health Professions The University of Texas MD Anderson Cancer Center Houston, TX, USA
Madhuri R. Hegde Division of Medical Genetics Department of Human Genetics Emory University Atlanta, GA, USA
Patrick Alan Lennon Clinical Cytogenetics Pathgroup, LLC Nashville, TN, USA
ISBN 978-1-4614-2169-6 e-ISBN 978-1-4614-2170-2 DOI 10.1007/978-1-4614-2170-2 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2012933788 © Springer Science+Business Media, LLC 2012 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com)
Preface
Clinical Molecular Diagnostics is an ever-expanding field which has come to the forefront of clinical testing with the increasing demand for molecular pathology and the ideologies of personalized medicine. It is apparent that with any rapid changes to a field, there is a learning curve. Our hope is to assist those who are either familiar or unfamiliar with Molecular Diagnostics in the areas of Infectious Disease, Oncology, Pre-/postnatal, and Human Identity Testing. Modern Clinical Molecular Techniques is divided into six parts. Part I focuses on current clinical molecular laboratory practices including the proper handling of specimens, DNA/RNA isolation, test validation, current general molecular techniques, summary reporting, and data storage. In Part II, we begin to take a more in-depth view on some of the more advanced protocols in clinical molecular infectious disease testing ranging from specimen identification to viral load testing using current diagnostic molecular techniques. Part III is focused on clinical molecular oncology testing. In this part, current clinical molecular diagnostic techniques are showcased in areas from detecting gene rearrangements, mutation analysis, cancer microarray
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