Molecular and morphophysiological characterization of superior cluster bean ( Cymopsis tetragonoloba ) varieties
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J. Crop Sci. Biotech. 2009 (September) 12 (3) : 143 ~ 148 DOI No. 10.1007/s12892-009-0106-8 RESEARCH ARTICLE
Molecular and Morphophysiological Characterization of Superior Cluster Bean (Cymopsis tetragonoloba) Varieties Anita Punia1, Rakesh Yadav2, Pooja Arora2, Ashok Chaudhury2 1 2
University Institute of Engineering and Technology, Kurukshetra University, Kurukshetra-136119 (Haryana), India Department of Bio and Nano Technology, Guru Jambheshwar University of Science & Technology, Hisar-125001 (Haryana), India
Received: July 9, 2009/ Revised September 17/ Accepted: September 21, 2009
Abstract Cluster bean (Cymopsis tetragonoloba) belongs to tribe Indigoferae of family Leguminosae. India is the world-leader for cluster bean production as it contributes 80% shares of its total production. Cluster bean (guar) is a cash crop for its application in textile, paper, petroleum, mining, pharmaceuticals, explosives, and food industries. Owing to its immense wealth of variable morphophysiological and industrial qualities there is a strong need for appropriate addressing and well documentation of the germplasm. Efforts are to be made to organize research programs on germplasm characterization, utilization, and molecular characterization. Superior cluster bean varieties were selected on the basis of morphophysiological characters and subjected to DNA-based molecular marker analysis. Eighteen of the best genotypes were chosen for DNA extraction, optimization of PCR conditions, and genetic diversity studies using 37 random primers. A total of 381 random amplification fragments were obtained; number of amplifications ranging from 4 to 22 with an average of 10.29 amplified fragments per primer. Evaluation of RAPD data reveals a magnificent range (0.34-0.76) of genotypic similarity coefficients. The UPGMA dendrogram was constructed based on similarity indices which illustrated discrete clustering of different genotypes into groups. Results recorded a positive correlation amongst varieties vis-à-vis cluster analysis generated by NTSYSpc and morphophysiological characteristics. The degree and distribution of genetic diversity in cluster bean would facilitate an evolutionary relationship between numerous accessions that eventually catalogues genetic resources in a more concerted fashion. Key words: Cluster analysis, DNA fingerprinting, genetic diversity, guar (Cymopsis tetragonoloba), RAPD
Introduction Cymopsis tetragonoloba (L.) Taub commonly known as guar is a cash crop of the family Leguminosae (Gillet 1958). It is a self-pollinated crop with 2n = 14 chromosomes (Ayyanagar and Krishnaswami 1933; Hymowitz and Upadhya 1963). It is widely cultivated in countries like India, Pakistan, USA, Italy, Morocco, Germany, Greece, Spain, and is thus considered as a new crop for western agricultural practices (Hymowitz and Matlock 1963). Guar seed is highly valued in numerous industries because of its galactomannan rich endosperm. Its nutritional and industrial value relies on one of the significant hydrocolloid products with high-thickeni
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