Morphology engineering: a new strategy to construct microbial cell factories
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(2020) 36:127
REVIEW
Morphology engineering: a new strategy to construct microbial cell factories Kaiyue Huo1 · Fengjie Zhao1 · Fang Zhang1 · Ruihua Liu2 · Chao Yang1 Received: 12 May 2020 / Accepted: 14 July 2020 © Springer Nature B.V. 2020
Abstract Currently, synthetic biology approaches have been developed for constructing microbial cell factories capable of efficient synthesis of high value-added products. Most studies have focused on the construction of novel biosynthetic pathways and their regulatory processes. Morphology engineering has recently been proposed as a novel strategy for constructing efficient microbial cell factories, which aims at controlling cell shape and cell division pattern by manipulating the cell morphologyrelated genes. Morphology engineering strategies have been exploited for improving bacterial growth rate, enlarging cell volume and simplifying downstream separation. This mini-review summarizes cell morphology-related proteins and their function, current advances in manipulation tools and strategies of morphology engineering, and practical applications of morphology engineering for enhanced production of intracellular product polyhydroxyalkanoate and extracellular products. Furthermore, current limitations and the future development direction using morphology engineering are proposed. Keywords Cell morphology-related genes · Extracellular products · Morphology engineering · Microbial cell factories · Polyhydroxyalkanoate
Introduction It is of great economic and scientific significance to construct microbial cell factories for producing biochemical products. In recent years, many efforts have been made to build efficient microbial cell factories to industrialize the target products, such as native metabolites, heterologous synthetic products and proteins (Davy et al. 2017). It aims to improve the fermentation capacity of industrial microorganisms and the physiological properties of cells for reducing the production cost, increasing the product output and expanding the product diversity. Supported by metabolic engineering and synthetic biology, more construction strategies of microbial cell factory have been developed * Ruihua Liu [email protected] * Chao Yang [email protected] 1
Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, Nankai University, Tianjin 300071, China
State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, China
2
mainly including chassis cell construction, synthetic pathway design and cell production performance optimization (Calero and Nikel 2019; Chae et al. 2017; Chi et al. 2019; Ramzi 2018). However, these strategies only focused on the genetic implants and regulatory processes, the features of microorganisms themselves, such as cell morphology, were rarely considered in the biological production process (Volke and Nikel 2018). Different bacteria have different cell shapes and structures to maintain specific lifestyles and perform specific physiological tasks in differen
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