Multi-directional Morphological Assessment of Single Bacterial Colonies Through Non-invasive Optical Imaging
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Annals of Biomedical Engineering (Ó 2020) https://doi.org/10.1007/s10439-020-02660-4
Original Article
Multi-directional Morphological Assessment of Single Bacterial Colonies Through Non-invasive Optical Imaging JUNSOO LEE,1 SEUNG-YEOL LEE,2 SANGYEOB HAN,1 DAEWOON SEONG,1 RUCHIRE ERANGA WIJESINGHE,3 PILUN KIM,4 MANSIK JEON,1 HEE-YOUNG JUNG,2 and JEEHYUN KIM1 1
School of Electronic and Electrical Engineering, Kyungpook National University, 80 Daehak-ro, Buk-gu, Taegu 41566, South Korea; 2School of Applied Biosciences, Kyungpook National University, 80 Daehak-ro, Buk-gu, Taegu 41566, South Korea; 3 Department of Biomedical Engineering, Kyungil University, 50 Gamasil-gil, Hayang-eup, Gyeongsan-si, Gyeongsangbukdo 38428, South Korea; and 4School of Medicine, Kyungpook National University, 80 Daehak-ro, Buk-gu, Taegu 41566, South Korea (Received 5 August 2020; accepted 12 October 2020) Associate Editor Daniel Elson oversaw the review of this article.
Abstract—A bacterium in bacterial colony is a basal component of bacterial studies and is therefore of considerable importance. The morphological characteristics of a single colony have been widely used as the standard to identify diverse bacterial species. However, the conventional methods for obtaining morphological information, such as microscopic techniques, cannot provide tomographic views. This study utilizes spectral-domain optical coherence tomography (SD-OCT) to observe both external and internal structures for single colonies. OCT imaging provided a clear top (projection) and lateral (cross-sectional) images, which can identify distinct features in the fifteen different bacterial species examined. Through the acquired OCT images, the elevation type in each genus was confirmed, and the quantitative measurements for morphology including a diameter, height, and elevation angle were achieved. Moreover, the OCT raw data was used to build three-dimensional images to observe outer appearances in all direction. As a result, the averaged angles of 7.92°, 14.40°, 6.78°, and 11.92° of the genus Hymenobacter, Spirosoma, Bacillus, and Deinococcus, respectively, were calculated. In conclusion, we have demonstrated that OCT is a highly feasible, noninvasive method for the identification of bacterial species, providing detailed morphological and visual information. It thus has strong potential for applications in bacteriology, as well as biomedical and food sciences.
Address correspondence to Jeehyun Kim, School of Electronic and Electrical Engineering, Kyungpook National University, 80 Daehak-ro, Buk-gu, Taegu 41566, South Korea. Electronic mail: [email protected] Jung, School of Applied Biosciences, Kyungpook National University, 80 Daehak-ro, Buk-gu, Taegu 41566, South Korea. Electronic mail: [email protected]
Keywords—Bacteriology, Single colony, Optical imaging, Tomographic view, Morphology.
INTRODUCTION Bacterial colonies represent macroscopic bodies that usually originate from a single cell, or a group of cells, belonging to a single species. Bacterial colony mo
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