Mutant Construction and Integration Vector-Mediated Genetic Complementation in Listeria monocytogenes
Genes that play a role in stress response mechanisms and other phenotypes of Listeria monocytogenes can be identified by construction and screening of mutant libraries. In this chapter, we describe the construction and screening of mutant libraries of L.
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Edward M. Fox Hélène Bierne Beatrix Stessl Editors
Listeria Monocytogenes Methods and Protocols Second Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, UK
For further volumes: http://www.springer.com/series/7651
For over 35 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-bystep fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice. These hallmark features were introduced by series editor Dr. John Walker and constitute the key ingredient in each and every volume of the Methods in Molecular Biology series. Tested and trusted, comprehensive and reliable, all protocols from the series are indexed in PubMed.
Listeria Monocytogenes Methods and Protocols Second Edition
Edited by
Edward M. Fox Department of Applied Sciences, Northumbria University, Newcastle-upon-Tyne, UK
Hélène Bierne Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France
Beatrix Stessl Institute of Food Safety, Food Technology and Veterinary Public Health, University of Veterinary Medicine Vienna, Vienna, Austria
Editors Edward M. Fox Department of Applied Sciences Northumbria University Newcastle-upon-Tyne, UK
He´le`ne Bierne Universite´ Paris-Saclay, INRAE, AgroParisTech Micalis Institute Jouy-en-Josas, France
Beatrix Stessl Institute of Food Safety Food Technology and Veterinary Public Health University of Veterinary Medicine Vienna Vienna, Austria
ISSN 1064-3745 ISSN 1940-6029 (electronic) ISBN 978-1-0716-0981-1 ISBN 978-1-0716-0982-8 (eBook) https://doi.org/10.1007/978-1-0716-0982-8 © Springer Science+Business Media, LLC, part of Springer Nature 2007, 2021 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and informat
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