Neural Cell Attachment on Metal Ion Implanted Glass Surfaces
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Neural Cell Attachment on Metal Ion Implanted Glass Surfaces Emel Sokullu-Urkac,1* Ahmet Oztarhan,1,2 Ismet Deliloglu-Gurhan,2 Sultan Gulce-Iz,2 Feyzan Ozdal-Kurt3 and Ian G. Brown4 1 Surface Modification Laboratory, Ege University, 35100 Izmir, Turkey 2 Bioengineering Department, Ege University, 35100 Izmir, Turkey 3 Biology Department, Celal Bayar University, 45040 Manisa, Turkey 4 Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA ABSTRACT We have explored the application of ion implantation as a tool for the enhancement of neural cell growth on glass surfaces. Glass substrates were ion implanted with gold and with carbon using a metal vapor vacuum arc (MEVVA) ion source-based implantation system at Ege University Surface Modification Laboratory. The implantation dose was varied over the range 1014 – 1017 ions/cm2 and the ion energy spanned the range 20 – 80 keV. B35 neural cells were seeded and incubated on the implanted substrates for 48h at 37°C. After 2-days in culture the cell attachment behavior was characterized using phase contrast microscopy. The adhesion and direct contact of neural cells on these ion implanted glass surfaces were observed. INTRODUCTION There is potential for the use of ion beam modification of surfaces for basic neurobiological research, with a variety of possibilities lying at the interface between inorganic and organic neuron systems, such as pain relief through neuron stimulation, regeneration of defective neurons, artificial retinas, and the realization of true neural computing [1-3]. Biocompatibility of the implant or substrate material is an important factor. The assessment of biocompatibility involves the ability of tissue to adhere to the surface of the material, and in general the more compatible the surface the greater the cell adhesion. In the work described here we have concentrated on the viability and morphology of neural cells on surfaces modified by ion implantation. The ion implantation was carried out over a range of implantation dose and ion energy, and cell culture experiments were done on all samples under the same condition. In this way, we were able to compare the cell attachment behavior on the modified surfaces. Our study covers cell attachment and network formation of neurons on Au- and Cimplanted glass surfaces. B35 neuroblastoma cell line was used for in vitro studies. B35 cells have proven useful model in the molecular analysis of endocytosis and of signaling pathways and in particular those that guide axonal outgrowth and cell motility [4]. In this study, we examined growth of cells in number in a culture medium with serum on ion implanted and unimplanted samples. Thus we examined the effect of ion implantation on neural cell adhesion and axonal outgrowth. EXPERIMENTAL DETAILS We used glass rather than polymer as the substrate material in order to eliminate any effects of polymeric interactions on the surfaces.
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Surface modification Glass substrates (microscope slides) were ion implanted using a vacuum arc ion sourcebased ion implantation
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