New biological labels based on functionalized YVO 4 :Eu nanoparticles
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AA6.2.1
New biological labels based on functionalized YVO4:Eu nanoparticles D. Giaume, V Buissette, K. Lahlil, T. Gacoin, J.-P. Boilot Laboratory of Condensed Matter Physics Ecole Polytechnique, route de Saclay, 91128 Palaiseau, France D. Casanova, E. Beaurepaire, M.-P. Sauviat, A. Mercuri, A. Alexandrou Laboratory for Optics and Biosciences, CNRS UMR 7645, INSERM U45L Ecole Polytechnique, route de Saclay, 91128 Palaiseau, France Abstract Lanthanide-ion doped oxide (YVO4:Eu) nanoparticles were synthesized as aqueous colloids and functionalized by a bioactive silane shell to be used as fluorescent biological labels. Nanoparticles functionalized with guanidinium groups were able to act as artificial toxins which specifically target Na+ channels. They were individually detectable in live cardiac myocytes. Functionalized oxide nanoparticles appear as a new interesting tool, especially attractive for long-term single-molecule tracking due to their photo-stability and long luminescence lifetime.
Introduction Fluorescent organic compounds have been extensively used for the visualization of the different components of biological systems.1,2,3 Now, an interesting issue is to track labeled individual species during in vitro or in vivo experiments. The use of organic dyes is limited for that purpose because of their rapid photobleaching. A few years ago, it was suggested that functionalized inorganic CdSe nanocrystals could be used,4,5 since they exhibit very good emission properties with the appropriate stability.6,7,8,9,10 Moreover, the emission wavelength can be tuned by modifying the size of the particles. In this paper, we discuss the application of a new promising class of biological fluorescent nanoparticles based on rare earth doped oxide nanocrystals. These compounds are characterized by high emission yields, at wavelengths that do not depend on the size of the particles, but which can be tuned by changing the chemical nature of the rare earth doping. Moreover, compared to CdSe particles, these systems do not exhibit any blinking of their emission since they are doped with a large number of emitting ions. Our study is focused on europium doped yttrium vanadate (YVO4:Eu) nanoparticles, with the aim to use them for the optical detection of Na+ channel dynamics on cell membranes. These channels are essential elements for electrical signaling in the nervous, muscular, and cardiac systems. Our goal is to have a direct detection of the mobility of these channels in the bilayered membrane of the cell, by tracking luminescent nanoparticles that are selectively bound to them. Our work is divided into four steps : i) to achieve the synthesis of the nanoparticles and their functionalization so as to ensure a specific interaction with the Na+ channels ii) to show the possibility of detection of individual particles iii) to test their bioactivity and iv) to visualize the nanoparticles in situ on cell membranes.
AA6.2.2
Synthesis and functionalization of the nanoparticles The colloidal synthesis of YVO4:Eu nanoparticles was de
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