Novel feasibilities for preparation of serum albumin-based core-shell nanoparticles in flow conditions
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Novel feasibilities for preparation of serum albumin-based core-shell nanoparticles in flow conditions Alexandra N. Kovács 1 & Norbert Varga 1 & Gyöngyi Gombár 1 & Viktória Hornok 1,2 & Edit Csapó 1,3 Received: 22 January 2020 / Accepted: 27 February 2020 # Akadémiai Kiadó 2020
Abstract We first demonstrate a simple and rapid fabrication protocol of bovine serum albumin (BSA) nanoparticles (NPs), as potential drug carriers using a microchannel flow technique with the successful encapsulation of a water-soluble kynurenic acid (KYNA) having neuroactive property has also been performed. By comparison, the preparation of a hydrophobic α-Tocopherol (TP)-loaded polylactide-co-glycolide 50:50 (PLGA50)-based NPs was also carried out under flow conditions. We highlight several benefits of the flow technique over the commonly known self-assembly and nanoprecipitation processes. The average particle diameter, the size distribution, the encapsulation efficiency (EE%) and the drug release kinetics of these different core-shell type NPs prepared by the flow as well as the above-mentioned classic methods were compared. The decisive role of the flow rate (FR), the relative flow rate (RFR) of the components in the particle size of both BSA- and PLGA50-based NPs have also been verified. By utilization of the optimal flow conditions, the average size can be decreased with ca. 15–20% and lower polydispersity index (PDI) can be also achieved. It was confirmed that the systematic change of the RFR values resulting in the controlled size of the drug-loaded BSA NPs between 120 and 140 nm, while d = 149 nm was obtained for self-assembled NPs. However, for BSA-based NPs quite similar EE% was obtained for both methods (ca. 11–12%), but for PLGA50/TP NPs the application of flow device increased the EE% from 67.0% to 71.5%. Keywords Serum albumin . PLGA . Kynurenic acid . Tocopherol . Core-shell nanocarriers . Microfluidic device
Introduction Several fabrication protocols are known in the literature for producing serum protein and polymeric (e.g. PLA, PLGA,
chitosan, alginate etc.) nanoparticles (NPs) as potential colloidal drug delivery systems (DDs) in the modern pharmaceutical researches. In case of serum albumin NPs, the commonly used preparation protocol is the desolvation process [1], where
Highlights - Controllable preparation of serum albumin nanocarriers containing kynurenic acid has been presented under flow conditions - By optimized flow conditions, a reproducible and large-scale production of BSA-based core-shell nanoparticles can be obtained - Utilization of flow device results in smaller particle sizes compared to self-assembly and nanoprecipitation methods Electronic supplementary material The online version of this article (https://doi.org/10.1007/s41981-020-00088-4) contains supplementary material, which is available to authorized users. * Viktória Hornok [email protected] * Edit Csapó [email protected] 1
Department of Physical Chemistry and Materials Science, Interdisciplinary Excelle
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