Novel nanoemulsion gel containing triple natural bio-actives combination of curcumin, thymoquinone, and resveratrol impr
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ORIGINAL ARTICLE
Novel nanoemulsion gel containing triple natural bio-actives combination of curcumin, thymoquinone, and resveratrol improves psoriasis therapy: in vitro and in vivo studies Karishma Khatoon 1 & Asgar Ali 1 & Fahan J. Ahmad 1 & Zubair Hafeez 2 & M. Moshahid A. Rizvi 2 & Sohail Akhter 1,3,4,5 & Sarwar Beg 1 Accepted: 7 September 2020 # Controlled Release Society 2020
Abstract Curcumin, resveratrol, and thymoquinone are the potential natural bio-actives reported with good anti-psoriatic activity. However, poor aqueous solubility and limited skin permeation of these natural bio-actives hinder their effective delivery and potential therapeutic outcome. In this regard, current research work focuses on the design and optimization of nanoemulsion (NE) gel formulation for the concurrent delivery of these three drugs. The NE system is consisting of oleic acid as oil phase, Tween 20 as surfactant, and PEG 200 as co-surfactant. The optimized formulation exhibited the droplet size 76.20 ± 1.67 nm, PDI of 0.12 ± 0.05, RI of 1.403 ± 0.007, and viscosity of 137.9 ± 4.07 mp. Carbopol 940 (0.5% w/v) was used as the gelling agent to prepare the NE gel which exhibited a good texture profile. The optimized formulation exhibited a higher % of growth inhibition on A-431 cells and demonstrated good anti-angiogenic activity in the HET-CAM test. Finally, in vivo studies in Balb/c mice model showed improved anti-psoriatic conditions which indicated that the triple natural bio-actives combination in nanoemulgel formulation is effective in the management of psoriasis. Keywords Nanoemulgel . Curcumin . Resveratrol . Thymoquinone . MTT assay . Anti-psoriatic assay
Introduction Psoriasis is an autoimmune disease involving the skin, and sometimes joints, bones, tendons, ligaments, nails, and mucosal membranes. It affects 79.7 million individuals worldwide, which corresponds to 2–4% of individuals. Psoriasis severity ranges from red patches to the whole body coverage [1]. The * Asgar Ali [email protected] 1
Nanomedicine Research Lab, Department of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi 110062, India
2
Department of Biosciences, Jamia Millia Islamia, New Delhi, India
3
Centre de Biophysique Moléculaire, CNRS UPR4301, Rue Charles Sadron, 45071 Orléans, Cedex 2, France
4
LE STUDIUM® Loire Valley Institute for Advanced Studies, Orléans, Centre-Val de Loire Region, France
5
Yousef Abdullatif Jameel Chair of Prophetic Medical Applications (YAJCPMA), Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia
standard treatment protocol of psoriasis leads to a reversal of skin to a clinically standard condition, i.e., by decreasing the response to the inflammatory cells and recovery of epidermal thickness [2]. The precise cause of this disorder is unknown; however, some factors including the environment, genetics, immune malfunction, and skin disorders such as cutting, trauma, and sting are responsible [3]. Although there are three treatment lines available for ps
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