Nuclear HSP90 and HSP70 in COPD patients treated with formoterol or formoterol and corticosteroids
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EUROPEAN JOURNAL OF MEDICAL RESEARCH
Eur J Med Res (2009) 14(Suppl. IV): 104-107
December 7, 2009 © I. Holzapfel Publishers 2009
NUCLEAR HSP90 AND HSP70 IN COPD PATIENTS TREATED WITH FORMOTEROL OR FORMOTEROL AND CORTICOSTEROIDS A. Holownia1, R. M. Mroz 2, A. Kielek1, E. Chyczewska 2, J. J. Braszko1
1 Department
of Clinical Pharmacology and 2 Department of Chest Diseases and Tuberculosis, Medical University of Bialystok, Poland
Abstract Objective: Heat shock proteins assist cellular protein folding and are required for the normal activity of steroid receptors. In this study we assessed nuclear HSP90 and HSP70 proteins and mRNA levels in cells isolated from induced sputum of chronic obstructive pulmonary disease patients treated for 4 weeks with formoterol (F) or formoterol+budesonide (F/ICS). Methods: Nuclear heat shock protein levels were assessed by Western blot and specific mRNAs were quantified in cell lysates using qRT-PCR. Results: Both HSP90 and HSP70 protein levels were higher in the F/ICS-treated patients in comparison with the F-treated group (by 31%, P
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