One-step procedure for peroxidase concentration, dye separation, and color removal by aqueous two-phase system
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RESEARCH ARTICLE
One-step procedure for peroxidase concentration, dye separation, and color removal by aqueous two-phase system Natalia Klanovicz 1,2 & Andressa Warken 2 & Letícia Paliga 2 & Aline Frumi Camargo 2 & Thamarys Scapini 2 & Jaqueline Garda Buffon 3 & Gislaine Fongaro 4 & Antonio Carlos Silva Costa Teixeira 1 & Helen Treichel 2 Received: 12 May 2020 / Accepted: 26 October 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract The aim of our study is to develop a one-step procedure to remove and degrade dyes from wastewater using a low-cost and efficient system based on aqueous two-phase system (ATPS), a well-known technique used to concentrate and recover enzymes. We investigated the catalytic proprieties of rice bran peroxidase (RBP) and found that this homemade enzyme can remain bound to its substrate for up to 5 days in controlled environments, without denaturing and while maintaining stable oxidation reduction potential (ORP) and pH. This biomolecule showed affinity for the ATPS technique prepared with polyethylene glycol and salt, which improved the relative activity up to 170%. The red dye separation in ATPS top phase was achieved in 3 min, in the RBP presence, with 100% of efficiency, and color removal of 87% was obtained in 24 h of enzymatic reaction. The process has promise to be scaled up to 10-fold and to reuse the reagents from the bottom phase of the ATPS. Keywords Dye extraction . Enzymatic-assisted treatment . Homemade enzyme . Reagent reuse . Scale-up . Wastewater decolorization
Introduction Dyes are widely present in industries and, as a consequence of water use in dye processing, large amounts of contaminated wastewater are generated. Dyes have detrimental effects in direct contact with hydric bodies, impacting and damaging ecosystem health (Husain 2010; Shakerian et al. 2020). To mitigate these problems, several countries instituted specific regulations regarding effluent discharges. Dye manufacturers are obligated to treat wastewater to achieve adequate Responsible editor: Tito Roberto Cadaval Jr * Helen Treichel [email protected] 1
Research Group in Advanced Oxidation Processes (AdOx), Department of Chemical Engineering, Escola Politécnica, University of São Paulo, São Paulo, Brazil
2
Laboratory of Microbiology and Bioprocess, Federal University of Fronteira Sul, Erechim, Brazil
3
Mycotoxin and Food Science Laboratory, School of Chemistry and Food, Federal University of Rio Grande, Rio Grande, Brazil
4
Laboratory of Virology, Federal University of Santa Catarina, Florianópolis, Brazil
pH, temperature, total dissolved solids, chemical oxygen demand, and color, among other parameters. The usual approach is to apply treatment processes such as flocculation, coagulation, decantation, and filtration, also known as primary, secondary, and tertiary treatments (Ghaly et al. 2014). Tertiary treatments involve the removal of dissolved compounds from wastewater, including dyes used in industrial processes. In conventional plant facilities, electrodialysis, r
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