Optimization for allitol production from d -glucose by using immobilized glucose isomerase and recombinant E. coli expre
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ORIGINAL RESEARCH PAPER
Optimization for allitol production from D-glucose by using immobilized glucose isomerase and recombinant E. coli expressing D-psicose-3-epimerase, ribitol dehydrogenase and formate dehydrogenase Xin Wen . Huibin Lin . Yilin Ren . Can Li . Chengjia Zhang . Xin Song . Jianqun Lin . Jianqiang Lin
Received: 1 March 2020 / Accepted: 17 May 2020 Ó Springer Nature B.V. 2020
Abstract Objective To develop a method combining enzymatic catalysis and resting-cell biotransformation to produce allitol from low cost substrate D-glucose. Results The recombinant E. coli expressing D-psicose-3-epimerase (DPE), ribitol dehydrogenase (RDH) and formate dehydrogenase (FDH) for allitol production from D-fructose was constructed. The optimizations of the cell catalytic conditions and the cell cultivation conditions were made. Then, 63.4 g allitol L-1 was obtained from 100 g D-fructose L-1 in 4 h catalyzed by the recombinant E. coli cells. In order to decrease the substrate cost, D-glucose was used as the substrate instead of D-fructose and immobilized glucose isomerase was used to convert D-glucose into
D-fructose. In order to simplify allitol production process from D-glucose, one-pot reaction using the mixed catalysts was used and the reaction conditions were optimized. Finally, 12.7 g allitol L-1 was obtained from 50 g D-glucose L-1 catalyzed by the mixed catalysts of immobilized glucose isomerase and the recombinant E. coli cells. Conclusions Allitol can be efficiently produced from low cost substrate D-glucose by using the method combining enzymatic catalysis and resting-cell biotransformation, which is the first report.
X. Wen C. Zhang X. Song (&) J. Lin J. Lin (&) State Key Laboratory of Microbial Technology, Shandong University (Qingdao), Qingdao, China e-mail: [email protected]
Introduction
J. Lin e-mail: [email protected] H. Lin Shandong Academy of Chinese Medicine, Jinan, China Y. Ren Qingdao Longding Biotech Limited Company, Qingdao, China C. Li School of Biological Engineering, Qilu University of Technology, Jinan, China
Keywords D-Glucose
Allitol Cofactor recycling D-Fructose Fed-batch culture Biotransformation
Allitol is a rare natural polyol containing six carbons atoms. It is a low-calorie sweetener and an important drug intermediate (Hassanin et al. 2016b). It crosslinks D- and L-hexoses and can be used for L-psicose biosynthesis (Izumori 2006). Allitol rarely exits in nature and extraction of allitol from natural materials will consume a lot of natural resources and do harm to the environments. Chemically synthesis of allitol is possible (Jumde et al. 2016), but the process is complicated and not environmental friendly. Fortunately, bioproduction
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of allitol by using enzyme or resting cell catalysis is possible. D-Psicose-3-epimerase (DPE) or D-tagatose3-epimerase (DTE) can transform D-fructose into Dpsicose, and ribitol dehydrogenase (RDH) can transform D-psicose into allitol consuming NADH, w
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