Osteogenic differentiation of BMSCs on MoS 2 composite nanofibers with different cell seeding densities
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ORIGINAL ARTICLE
Osteogenic differentiation of BMSCs on MoS2 composite nanofibers with different cell seeding densities Shulu Luo1 · Shuyi Wu1 · Jianmeng Xu1 · Xingcai Zhang2 · Leiyan Zou1 · Run Yao1 · Lin Jin3 · Yan Li1 Received: 12 April 2020 / Accepted: 30 May 2020 © King Abdulaziz City for Science and Technology 2020
Abstract Molybdenum disulphide (MoS2) exhibits unique properties that are useful for various biomedical applications. Owing to its distinct characteristics and osteogenic differentiation promotion effect, this material has been studied extensively. However, the effect of cell density on osteogenic differentiation between M oS2-based biomaterials and cells is still unknown. In this study, we used MoS2/polyacrylonitrile (PAN) composite nanofibres as substrates to evaluate the effect of cellular density on the osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs). We created different experimental groups with increasing cell seeding densities and investigated cellular behaviours, biocompatibility, proliferation and osteogenic properties. The results show that MoS2/PAN composite nanofibres can positively regulate osteogenic differentiation. More importantly, in the presence of standard culture conditions, 1.0 × 104 cells/cm2 is the most efficient and suitable cell seeding density for BMSCs osteogenic differentiation. Our findings suggest that the optimal cell density for osteogenesis is vital for the osteogenic differentiation of BMSCs when these cells are cultured onto MoS2-based biomaterials. Keywords Molybdenum disulphide · Nanofibers · Cell seeding density · Osteogenesis
Introduction Molybdenum disulphide ( MoS2) is a two-dimensional (2D) material and a typical component of the layered transition metal dichalcogenides (TMDs) (Wang et al. 2018). This graphene-like structured compound exhibits unique physical, chemical, electronic, and mechanical properties that are Shulu Luo and Shuyi Wu contributed equally. * Lin Jin [email protected] * Yan Li [email protected] 1
Guangdong Provincial Key Laboratory of Stomatology, Department of Prosthodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-Sen University, No. 56 Lingyuan Road, Guangzhou 510055, People’s Republic of China
2
John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA
3
International Joint Research Laboratory for Biomedical Nanomaterials of Henan, Zhoukou Normal University, No. 6, Wenchang Road, Zhoukou 466001, People’s Republic of China
useful in various fields, ranging from electronics to biomedicine, such as bioelectronics, cancer therapy, antibacterial effects, and stem cell differentiation (Choi et al. 2017; Luo et al. 2018; Suhito et al. 2017; Wang et al. 2015; Yuan et al. 2019). MoS2 has already been explored extensively in the field of chemical catalysis and sensors. However, its applications in the biomedical field have been limited (Wang et al. 2015). Recently, MoS2 has been garnering increasing atte
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