Osteopontin Regulates Endometrial Stromal Cell Migration in Endometriosis through the PI3K Pathway
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ORIGINAL ARTICLE
Osteopontin Regulates Endometrial Stromal Cell Migration in Endometriosis through the PI3K Pathway Osteopontin Regulates Endometrial Cell Migration in Endometriosis Xiaoxia Fu 1 & Mengyun Yao 2 & Chaoshuang Ye 1 & Tao Fang 1 & Ruijin Wu 1 Received: 20 April 2020 / Accepted: 17 August 2020 # The Author(s) 2020
Abstract Endometriosis is generally characterized as a tumor-like disease because of its potential for distant metastasis and local tissue invasion, while whether osteopontin (OPN) plays a role in the pathogenesis of endometriosis has not been thoroughly investigated. We investigated the expression of OPN, urokinase plasminogen activator (uPA), phosphatidylinositol 3 kinase (PI3K), and phospho-PI3 kinase (p-PI3K) in endometrial stromal cells (ESCs). The serum concentration of OPN was determined by enzyme-linked immunosorbent assays (ELISA). OPN was downregulated to explore the corresponding change of uPA, p-PI3K, F-actin, and α-tubulin. The expression of OPN, uPA, PI3K, and p-PI3K was evaluated by western blot and quantitative real-time PCR (RT-qPCR) and the expression of F-actin and α-tubulin was confirmed by immunofluorescence assay. The proliferation and migration abilities of ESCs were investigated by CCK8, transwell, and wound scratch assays. Endometrial OPN, p-PI3K, and uPA expressions and serum OPN levels were increased in patients with endometriosis compared with the control. The expressions of p-PI3K, uPA, and α-tubulin were decreased by siRNA-OPN interference in ectopic ESCs. Activation and inhibition of the PI3K pathway apparently upregulate and downregulate uPA expression. Knockdown of OPN and inhibition of the PI3K pathway remarkably inhibited cell migration in ectopic ESCs. Meanwhile, activation of the PI3K pathway promoted the migration ability of ectopic ESCs. OPN may regulate the expression of uPA through the PI3K signal pathway to affect the migration ability of ESCs, indicating that OPN, uPA, and the PI3K pathway may be potential targets for interrupting development of endometriosis. Keywords Osteopontin . endometriosis . cell migration . PI3K . uPA
Abbreviations PI3K Phosphatidylinositol 3 kinase uPA Urokinase plasminogen activator
I would like to declare on behalf of my co-authors that the work described was original that has not been published previously, and not under consideration for publication elsewhere, in whole or in part. * Ruijin Wu [email protected] 1
Department of Obstetrics and Gynecology, Women’s Hospital, Zhejiang University School of Medicine, Hangzhou 310006, Zhejiang Province, People’s Republic of China
2
Institute of Burn Research, South-West Hospital, State Key Lab of Trauma, Burn and Combined Injury, Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University, Chongqing, China
Introduction Endometriosis, defined as the implantation and periodic growth of endometrial glands and stroma at extra-uterine sites, is a common benign gynecological disease with a heavy social and economic burden since symptoms of endometriosis i
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