Physicochemical characteristics of the active fractions of polyphenols from Arctic macrophytes
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Physicochemical characteristics of the active fractions of polyphenols from Arctic macrophytes Konstantin Bogolitsyn 1,2 & Anastasia Parshina 1 & Anna Druzhinina 1,2 & Denis Ovchinnikov 1 & Valeriy Krasikov 3 & Sergey Khviyuzov 2 Received: 5 March 2020 / Revised and accepted: 10 August 2020 # Springer Nature B.V. 2020
Abstract Brown algae of the Fucaceae family, collected in the western segment of the Arctic, contain a large number of polyphenolic compounds—phlorotannins—with a unique chemical nature, macromolecular structure, and physicochemical properties, exhibiting bioactive properties. The peculiarity of the biopolymers’ properties and structure, which are an element of fundamental scientific research of the cycle, structure—functional nature—properties, determines the priority of the directions of use of polyphenols as effective biologically active pharmaceutical preparations. This work aims to develop methods for selective extraction of phlorotannins from the Arctic brown alga Fucus vesiculosus, characterization of physicochemical properties, and biological activity of these compounds. The efficiency of solid-phase extraction (SPE) on a hydrophobic sorbent with grafted hexadecyl groups was shown for fractionation of the polyphenolic extract: active fractions were obtained, characterized by a high content of polyphenols (yield of polyphenols up to 98% relative to the content of these components in algae) with mass-average molecular weights of 21.4–35.8 kDa and oligomeric components in the mass range 374–994 Da. The IR spectra of the fractions were characterized, and the tendency of phlorotannins to form aggregates at high concentrations of the extract was shown. During hydrodynamic studies, a strong polyelectrolyte swelling of polyphenols (PP) was noted. The obtained polyphenolic fractions differ in physicochemical characteristics (acid-base and redox properties) and antioxidant activity. Keywords Phaeophyceae . Polyphenols . Phlorotannins . Solid-phase extraction . Physicochemical properties . HPLC-MS . Redox potential . Acid-base properties . Antioxidant activity
Introduction Brown algae are a unique raw material for a variety of substances with a wide range of consumer properties. The component composition of Arctic brown algae includes polyphenolic compounds—phlorotannins (polymers of phloroglucinol)— with content in the biomass reaching 20% (Ragan and Glombitza 1986). According to the structure and polymer properties, phlorotannins represent an extensive group of molecules * Anna Druzhinina [email protected] 1
Northern (Arctic) Federal University named after M.V. Lomonosov, Northern Dvina Emb. 17, Arkhangelsk 163002, Russia
2
N. Laverov Federal Center for Integrated Arctic Research of the Ural Branch of the Russian Academy of Sciences, Northern Dvina Emb. 23, Arkhangelsk 163000, Russia
3
Institute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoy pr. 31, Saint-Petersburg 199004, Russia
that differ in the degree of polymerization, the nature of the bonds between the phloro
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