Plasma circulating miR-23~27~24 clusters correlate with the immunometabolic derangement and predict C-peptide loss in ch
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ARTICLE
Plasma circulating miR-23~27~24 clusters correlate with the immunometabolic derangement and predict C-peptide loss in children with type 1 diabetes Silvia Garavelli 1,2 & Sara Bruzzaniti 2,3 & Elena Tagliabue 1 & Dario Di Silvestre 4 & Francesco Prattichizzo 1 & Enza Mozzillo 5 & Valentina Fattorusso 5 & Lucia La Sala 1 & Antonio Ceriello 1 & Annibale A. Puca 1,6 & Pierluigi Mauri 4 & Rocky Strollo 7 & Marco Marigliano 8 & Claudio Maffeis 8 & Alessandra Petrelli 9 & Emanuele Bosi 9,10 & Adriana Franzese 5 & Mario Galgani 2,11 & Giuseppe Matarese 2,11 & Paola de Candia 1 Received: 14 April 2020 / Accepted: 15 June 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Aims/hypothesis We aimed to analyse the association between plasma circulating microRNAs (miRNAs) and the immunometabolic profile in children with type 1 diabetes and to identify a composite signature of miRNAs/immunometabolic factors able to predict type 1 diabetes progression. Methods Plasma samples were obtained from children at diagnosis of type 1 diabetes (n = 88) and at 12 (n = 32) and 24 (n = 30) months after disease onset and from healthy control children with similar sex and age distribution (n = 47). We quantified 60 robustly expressed plasma circulating miRNAs by quantitative RT-PCR and nine plasma immunometabolic factors with a recognised role at the interface of metabolic and immune alterations in type 1 diabetes. Based on fasting C-peptide loss over time, children with type 1 diabetes were stratified into the following groups: those who had lost >90% of C-peptide compared with diagnosis level; those who had lost
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