Porcine Immunoglobulin Fc Fused P30/P54 Protein of African Swine Fever Virus Displaying on Surface of S. cerevisiae Elic
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RESEARCH ARTICLE
Porcine Immunoglobulin Fc Fused P30/P54 Protein of African Swine Fever Virus Displaying on Surface of S. cerevisiae Elicit Strong Antibody Production in Swine Chen Chen1 • Deping Hua1 • Jingxuan Shi1 • Zheng Tan1 • Min Zhu1 • Kun Tan1 • Lilin Zhang1 Jinhai Huang1
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Received: 6 December 2019 / Accepted: 13 July 2020 Wuhan Institute of Virology, CAS 2020
Abstract African swine fever virus (ASFV) infects domestic pigs and European wild boars with strong, hemorrhagic and high mortality. The primary cellular targets of ASFV is the porcine macrophages. Up to now, no commercial vaccine or effective treatment available to control the disease. In this study, three recombinant Saccharomyces cerevisiae (S. cerevisiae) strains expressing fused ASFV proteins-porcine Ig heavy chains were constructed and the immunogenicity of the S. cerevisiae-vectored cocktail ASFV feeding vaccine was further evaluated. To be specific, the P30-Fcc and P54-Fca fusion proteins displaying on surface of S. cerevisiae cells were produced by fusing the Fc fragment of porcine immunoglobulin IgG1 or IgA1 with p30 or p54 gene of ASFV respectively. The recombinant P30-Fcc and P54-Fca fusion proteins expressed by S. cerevisiae were verified by Western blotting, flow cytometry and immunofluorescence assay. Porcine immunoglobulin Fc fragment fused P30/P54 proteins elicited P30/P54-specific antibody production and induced higher mucosal immunity in swine. The absorption and phagocytosis of recombinant S. cerevisiae strains in IPEC-J2 cells or porcine alveolar macrophage (PAM) cells were significantly enhanced, too. Here, we introduce a kind of cheap and safe oral S. cerevisiae-vectored vaccine, which could activate the specific mucosal immunity for controlling ASFV infection. Keywords African swine fever virus (ASFV) S. cerevisiae Porcine immunoglobulin Fc P30-Fcc/P54-Fca fusion proteins
Introduction African swine fever (ASF) is a potent infectious disease caused by ASFV, leading to 100% mortality in acute infections. Since the first report of ASF in Kenya in 1921, dozens of countries and regions have erupted already (Luther et al. 2008). China announced the first ASF epidemic in August 2018, and multiple outbreaks had been reported currently (Zhao et al. 2019). ASFV had posed a Chen Chen and Deping Hua have contributed equally to this work.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12250-020-00278-3) contains supplementary material, which is available to authorized users. & Jinhai Huang [email protected] 1
School of Life Sciences, Tianjin University, Tianjin 300072, China
significant threat to the swine industry worldwide, causing deadly substantial economic losses. ASFV targets porcine macrophages via clathrin and cholesterol-dependent endocytosis (Galindo et al. 2015) to initiate infection, leading to high fever, severe depression, anorexia, skin redness, congestion, cyanosis, peripheral
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