Potentiometric sensing of histamine using immobilized enzymes on layered double hydroxides
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ORIGINAL ARTICLE
Potentiometric sensing of histamine using immobilized enzymes on layered double hydroxides Slah Hidouri1 • Abdel Hamid Errachid2,3 • Joan Baussels4 • Yaroslav I. Korpan5 Oscar Ruiz-Sanchez6 • Zouhair M. Baccar1
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Revised: 8 September 2020 / Accepted: 15 September 2020 Ó Association of Food Scientists & Technologists (India) 2020
Abstract Diamine oxydase and peroxidase have been coimmobilized onto layered double hydroxide (LDH) thin films for the development of real-time histamine biosensors. The chosen LDH materials are Mg2AlCO3, Mg4FeCl and Ca2AlCl. Prepared bi-enzymatic hybrid nanomaterials are capable of detecting histamine through the electrochemical oxidation of H2O2 and are used as the sensitive membrane for potentiometric microelectrode. Histamine biosensors developed in this work have fast response of less than 20 s, are sensitive and selective, with a large dynamic range of 10–8–10–3 M and a limit of detection of less than 10–8 M. The detection limit of the developed bienzymatic biosensors is relatively higher than those corresponding with gas and liquid chromatography, which are still considered as the reference methods. Finally, the reproducibility, the specificity and the storage stability of the biosensors were studied. & Slah Hidouri [email protected] 1
Nanobioengineering Group, National Institute for Research and Physicochemical Analysis (INRAP), Biotechpoˆle de Sidi Tabet, 2020 Sidi Thabet, Ariana, Tunisia
2
Laboratory of Nanobioinge´niery-IBEC, Scientific Parc of Barcelona (PCB), Barcelona, Spain
3
Laboratory of Analytical Sciences (LSA)-UMR 5180, University Claude Barnard of (UCB) Lyon I, Villeurbanne, France
4
CSIC - Instituto de Microelectronica de Barcelona (IMBCNM), Barcelona, Spain
5
Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Str, Kyiv-143 03680-UA, Ukraine
6
Laboratory of Electronic, Faculty of Physics, University of Barcelone (UB), Barcelone, Spain
Keywords Potentiometric biosensor Histamine Hybrid nanomaterial Immobilization Layer double hydroxide Specificity
Introduction Biogenic amines (histamine, putrescine, cadaverine) are low-molecular weight organic compounds present in various foods particularly in fish and fish products, cheese, meat and wine (Razavi-Rohani et al. 2013; Biji et al. 2016). Histamine is related to food poisoning while putrescine and cadaverine are not toxic, but they increase the toxicity of histamine (Tortorella et al. 2014; Del Rio et al. 2019). The food intoxication related to histamine consumption is due to higher activity of decarboxylase enzymes (Landete et al. 2008). As described by Taylor and Eitenmiller (1986), a high dose of histamine reduces the capacity of the intestinal tract of human to detoxify histamine that enters bloodstream and causes intoxication (Visciano et al. 2014; Maintz and Novak 2007). However, a low level of biogenic amines and especially histamine is an indicator of a high level of food quality and hygienic processing (Prester 2011
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