Primary neuron and astrocyte cultures from postnatal Callithrix jacchus : a non-human primate in vitro model for researc
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Primary neuron and astrocyte cultures from postnatal Callithrix jacchus: a non-human primate in vitro model for research in neuroscience, nervous system aging, and neurological diseases of aging Angela O. Dorigatti & Stacy A. Hussong & Stephen F. Hernandez & Aubrey M. Sills & Adam B. Salmon & Veronica Galvan Received: 25 July 2020 / Accepted: 8 October 2020 # American Aging Association 2020
Abstract The ability to generate in vitro cultures of neuronal cells has been instrumental in advancing our understanding of the nervous system. Rodent models have been the principal source of brain cells used in Angela O. Dorigatti and Stacy A. Hussong contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11357-020-00284-z) contains supplementary material, which is available to authorized users. A. O. Dorigatti : S. A. Hussong : S. F. Hernandez : V. Galvan (*) Department of Cellular and Integrative Physiology, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, STCBM 3.200.8, San Antonio, TX 78245, USA e-mail: [email protected] A. O. Dorigatti : S. A. Hussong : S. F. Hernandez : A. M. Sills : A. B. Salmon : V. Galvan Barshop Institute for Longevity and Aging Studies, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA S. A. Hussong : A. B. Salmon : V. Galvan South Texas Veterans Health Care System, San Antonio, TX, USA A. B. Salmon Department of Molecular Medicine, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA V. Galvan Glenn Biggs Institute for Alzheimer’s & Neurodegenerative Diseases, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA
primary cultures for over a century, providing insights that are widely applicable to human diseases. However, therapeutic agents that showed benefit in rodent models, particularly those pertaining to aging and age-associated dementias, have frequently failed in clinical trials. This discrepancy established a potential “translational gap” between human and rodent studies that may at least partially be explained by the phylogenetic distance between rodent and primate species. Several non-human primate (NHP) species, including the common marmoset (Callithrix jacchus), have been used extensively in neuroscience research, but in contrast to rodent models, practical approaches to the generation of primary cell culture systems amenable to molecular studies that can inform in vivo studies are lacking. Marmosets are a powerful model in biomedical research and particularly in studies of aging and age-associated diseases because they exhibit an aging phenotype similar to humans. Here, we report a practical method to culture primary marmoset neurons and astrocytes from brains of medically euthanized postnatal day 0 (P0) marmoset newborns that yield highly pure primary neuron and astrocyte cultures. Primary marmoset neuron and astrocyte cultures can be generated reliably to provide a powerful NHP in vit
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