Prokaryotic Communities in Saline Soils of the Lake Elton Area in a Soil Catena along the Khara River
- PDF / 1,662,665 Bytes
- 15 Pages / 612 x 792 pts (letter) Page_size
- 38 Downloads / 225 Views
RIMENTAL ARTICLES
Prokaryotic Communities in Saline Soils of the Lake Elton Area in a Soil Catena along the Khara River A. I. Kuznetsovaa, *, E. A. Ivanovab, O. S. Samylinaa, F. G. Kurbanovac, D. S. Gruzdevd, T. A. Kanapatskiya, and N. V. Pimenova aWinogradsky
Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, 119071 Russia b Dokuchaev Soil Science Institute, Moscow, 119017 Russia cFaculty of Soil Science, Moscow State University, Moscow, 119991 Russia d Bioengineering Center, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, 117312 Russia *e-mail: [email protected] Received June 7, 2020; revised July 12, 2020; accepted July 17, 2020
Abstract—Analysis of the taxonomic structure of prokaryotic microbial complexes of the saline soils in the Lake Elton area and their comparative characteristics were carried out for a soil catena along the Khara River flowing into the hypersaline Lake Elton. High-throughput pyrosequencing detected 11 bacterial and 2 archaeal phyla. Comparative analysis of community structure revealed the differences between Solonchaks located at the river flood land and Kastanozems at the slope and top of the river terrace. The patterns of occurrence were described for six predominant phyla (Euryarchaeota, Thaumarchaeota, Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria). For the floodplain soils, the relations between the distribution of Euryarchaeota, Thaumarchaeota, Firmicutes, Actinobacteria, Proteobacteria and soil salinity as well as soil pH were described. Occurrence of members of the phyla Euryarchaeota, Thaumarchaeota, Firmicutes, Actinobacteria, and Proteobacteria in flood land soils was shown to depend on the soil salinity and pH. Members of the phyla Actinobacteria and Firmicutes were shown to predominate in Gleyic Kastanozems . Cloning of the 16S rRNA gene revealed high diversity within the genus Streptomyces (Actinobacteria). A number of the isolated streptomycete clones were homologous to the known antibiotic producers, which indicates that soils of this area are promising for further biotechnological screening. Keywords: microbial diversity, saline soils, arid ecosystems, Streptomyces, Lake Elton, high-throughput sequencing pyrosequencing, microbial biotechnology DOI: 10.1134/S0026261720060119
Microbial diversity in the communities of dry and saline habitats is of special interest both in relation to the problems of global climate changes and for assessment of the biotechnological utility of these habitats. Global climate change and increasing anthropogenic load result in desertification and higher shares of saline soils in natural ecosystems worldwide (Rengasamy, 2006). The presently urgent tasks are investigation of succession of microbial communities under conditions of arid climate and of changes in the microbiome composition, ecological functions, and mechanisms of adaptation to changing regimes of salinization, alkalization, plant cover, and other characteristics of the soils of dry habitats.
Data Loading...
