Promoter DNA Hypermethylation of the Cysteine Dioxygenase 1 ( CDO1 ) Gene in Intraductal Papillary Mucinous Neoplasm (IP
- PDF / 2,026,773 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 24 Downloads / 253 Views
ORIGINAL ARTICLE – TRANSLATIONAL RESEARCH AND BIOMARKERS
Promoter DNA Hypermethylation of the Cysteine Dioxygenase 1 (CDO1) Gene in Intraductal Papillary Mucinous Neoplasm (IPMN) Yoshiki Fujiyama, MD1, Yusuke Kumamoto, MD, PhD1, Nobuyuki Nishizawa, MD, PhD1, Shuji Nakamoto, MD1, Hiroki Harada, MD1, Kazuko Yokota, MD1, Yoko Tanaka, MD1, Kazuharu Igarashi, MD, PhD1, Hironobu Oiki, MD1, Kosuke Okuwaki, MD, PhD2, Tomohisa Iwai, MD, PhD2, Sabine Kajita, MD, PhD3, Hiroyuki Takahashi, MD, PhD3, Hiroshi Tajima, MD1, Takashi Kaizu, MD, PhD1, Jiichiro Sasaki, MD, PhD4, Masahiko Watanabe, MD, PhD, FACS1, and Keishi Yamashita, MD, PhD, FACS1,5 1
Department of Surgery, Kitasato University School of Medicine, Sagamihara, Kanagawa , Japan; 2Department of Gastroenterology, Kitasato University School of Medicine, Sagamihara, Kanagawa , Japan; 3Department of Pathology, Kitasato University School of Medicine, Sagamihara, Kanagawa , Japan; 4Multidisciplinary Cancer Care and Treatment Center, Kitasato University School of Medicine, Sagamihara, Kanagawa , Japan; 5Division of Advanced Surgical Oncology, Research and Development Center for New Medical Frontiers, Kitasato University School of Medicine, Sagamihara, Kanagawa , Japan
ABSTRACT Background. Intraductal papillary mucinous neoplasm (IPMN) involves adenoma (IPMA), a precancerous lesion, cancer (IPMC) including high-grade dysplasia (HGD), and invasive carcinoma (IC). DNA markers of IPMN are required for detection of invasive disease, and cysteine dioxygenase 1 (CDO1) gene promoter hypermethylation is a potential candidate. However, it has never been investigated in the context of IPMN. Patients and Methods. A total of 107 IPMN tumor tissues, including 41 IPMC and 66 IPMA, were studied. CDO1 promoter methylation was quantified using TaqMan quantitative methylation-specific polymerase chain reaction (qMSP) in patients with IPMN and other pancreatic cystic disorders after pancreatectomy. Results. The methylation values (TaqMeth Vs) of CDO1 increased when noncancerous pancreas tissues were compared with IPMA and HGD (p \ 0.0001). Among IPMC, the TaqMeth Vs in IC were not significantly higher than in HGD. The TaqMeth Vs of the solid tumors were higher
Ó Society of Surgical Oncology 2020 First Received: 20 April 2019 K. Yamashita, MD, PhD, FACS e-mail: [email protected]
than those of the cystic tumors (p = 0.0016), which were in turn higher than the corresponding noncancerous tissues (p \ 0.0001). Prognostic analysis revealed that high TaqMeth Vs (C 14.1) resulted in a poorer prognosis than low TaqMeth Vs (\ 14.1) (p \ 0.0001). In other pancreatic cystic diseases, only malignant mucinous cystic neoplasm showed DNA hypermethylation of its promoter. A pilot study in pancreatic juice confirmed methylation in all IPMN samples but not in benign pancreatic diseases (p = 0.0277). Conclusions. CDO1 promoter hypermethylation is extremely specific to IPMN and may accumulate with IPMN tumor progression during the adenoma–carcinoma sequence. It might be a promising candidate as a dia
Data Loading...
