Promoting Osseointegration of Dental Implants in Dog Maxillary Sinus Floor Augmentation Using Dentin Matrix Protein 1-Tr
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ORIGINAL ARTICLE
Promoting Osseointegration of Dental Implants in Dog Maxillary Sinus Floor Augmentation Using Dentin Matrix Protein 1-Transduced Bone Marrow Stem Cells Dong Ma1 • Yuanyin Wang1 • Yongxiang Chen1 • Gang Yang1 • Xin Liu1,2
Received: 15 March 2020 / Revised: 29 April 2020 / Accepted: 22 May 2020 Ó The Korean Tissue Engineering and Regenerative Medicine Society 2020
Abstract BACKGROUND: Beta-tricalcium phosphate (b-TCP) has been employed successfully as a synthetic graft material in maxillary sinus floor augmentation (MSFA) for placing dental implants. However, the lack of osteogenic and osteoinductive properties of this substitute invariably results in bone regeneration of low quality and quantity. The purpose of this study was to determine whether loading dentin matrix protein-1 (DMP1) gene-modified bone marrow mesenchymal stem cells (BMSCs) onto b-TCP promoted bone regeneration and osteointegration of dental implants in MSFA of dogs. METHODS: BMSCs were transduced with a lentiviral vector overexpressing the DMP1 gene (Lenti-DMP1) and with a lentiviral vector overexpressing enhanced green fluorescent protein (Lenti-EGFP) in vitro and were loaded into b-TCP scaffolds for autologous sinus grafting. Beagles received bilateral MSFA with four biomaterials ( Lenti-DMP1-transduced BMSCs/b-TCP, ` Lenti-EGFP-transduced BMSCs/b-TCP, ´ BMSCs/b-TCP, ˆ b-TCP) and simultaneous implant placement at each sinus. Twelve weeks post operation, the maxillae were explanted, and every sinus was evaluated by radiographic observation, micro-CT and histological analysis. The osteogenic outcomes of bone regeneration and osseointegration were compared between the four groups. RESULTS: The sinuses grafted with Lenti-DMP1-transduced BMSCs/b-TCP constructs presented a significantly higher increase in compact radiopaque area, higher local bone mineral densities, greater bone-implant contact and greater bone density when compared to other three groups. CONCLUSION: These results demonstrated that combinations of b-TCP and DMP1 gene-modified BMSCs could be used to construct tissue-engineered bone to enhance mineralization of the regenerated bone and osseointegration of dental implants in MSFA. Keywords Bone marrow mesenchymal stem cells Dentin matrix protein-1 Oral implant
1 Introduction
& Xin Liu [email protected] 1
Department of Oral and Maxillofacial Center, Key Laboratory of Oral Diseases Research of Anhui Province, Stomatologic Hospital and College, Anhui Medical University, Hefei 230032, China
2
Oral and Maxillofacial Surgery, Key Laboratory of Oral Diseases Research of Anhui Province, Stomatologic Hospital and College, Anhui Medical University, Hefei 230032, China
In recent decades, titanium dental implants have been demonstrated to be an effective tool to support prostheses and have been widely used to restore missing teeth. Alveolar resorption and continued pneumatization of the sinus after loss of teeth in the posterior maxilla invariably result in an inadequate amount of bone for a succes
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