Prospects of next generation sequencing in lentil breeding
- PDF / 749,447 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 12 Downloads / 209 Views
REVIEW
Prospects of next generation sequencing in lentil breeding Jitendra Kumar1 · Debjyoti Sen Gupta1 Received: 6 June 2020 / Accepted: 3 October 2020 © Springer Nature B.V. 2020
Abstract Lentil is an important food legume crop that has large and complex genome. During past years, considerable attention has been given on the use of next generation sequencing for enriching the genomic resources including identification of SSR and SNP markers, development of unigenes, transcripts, and identification of candidate genes for biotic and abiotic stresses, analysis of genetic diversity and identification of genes/ QTLs for agronomically important traits. However, in other crops including pulses, next generation sequencing has revolutionized the genomic research and helped in genomic assisted breeding rapidly and cost effectively. The present review discuss current status and future prospects of the use NGS based breeding in lentil. Keywords DNA sequencing · Functional markers · Unigenes · Transcriptome · Genetic gain · Pan-genome · Lentil
Introduction Lentil (Lens culinaris Medik.) is a diploid (2n = 14) selfpollinated crop and has a genome size of 4 Gbp [1]. It is a cool season crop that is cultivated globally in more than 52 countries. It is one of the healthiest pulse crops because its seeds are rich in minerals, fibers, and carbohydrates besides proteins (22–35%). During the past years significant increase has been observed in the productivity of lentil due to cultivation of high yielding varieties developed through traditional breeding. However, poor competitive ability to weeds, higher flower drop rate, pod shedding, and several biotic and abiotic stresses are still causes of reduced productivity in lentil crops [2]. Moreover, environmental conditions have high impact on the expression of agronomically important quantitative traits leading to poor genetic gain in lentil [3]. This is challenging to overcome under current climatic conditions. However, genomics has provided a potential way to increase the genetic gain in lentil and hence significant progress has been made in the past years for developing genomic resources in lentil. These genomic resources include availability of genome sequences, transcriptome sequences, molecular markers (i.e. SNPs and * Jitendra Kumar [email protected] 1
Division of Crop Improvement, ICAR-Indian Institute of Pulses Research, Kalyanpur, Kanpur 208024, India
SSRs), mapping populations, markers linked to genes/QTLs controlling important traits [4–10]. Further, advanced next generation sequencing technologies have accelerated the development of genomic resources rapidly and cost effectively and their use enriched genomic resources in different crops including orphan legume crops. Thus genomically poor legume crops have become rich with genomic resources due to the availability of draft genome sequences [11, 12] and NGS based approaches. For example, QTL-Seq made possible to identify genes/QTLs rapidly in different legume crops including chickpea, pigeonpea and grou
Data Loading...
