Protective effects of l -theanine on rats with dextran sulfate sodium-induced inflammatory bowel disease
- PDF / 4,828,346 Bytes
- 42 Pages / 595.276 x 790.866 pts Page_size
- 96 Downloads / 197 Views
Online ISSN 1976-3786 Print ISSN 0253-6269
RESEARCH ARTICLE
Protective effects of l‑theanine on rats with dextran sulfate sodium‑induced inflammatory bowel disease Ling Chen1,2 · Wen‑jun Xiao1,2 · Qiong‑xian Yan3 · Zhi‑hua Gong1,2 · Sheng Zhang1,2 · Li Zeng1,2,4 · Ming Yang5 · Yan‑he Zhou6
Received: 16 December 2019 / Accepted: 29 June 2020 © The Pharmaceutical Society of Korea 2020
Abstract The aim of this study is to evaluate the antiinflammatory and protective effects of l-theanine in inflammatory bowel disease (IBD) and to identify the underlying molecular mechanisms. Rats were pre-treated with l-theanine at 0, 50, 200, or 800 mg/kg/day. IBD was induced in rats using dextran sulfate sodium (DSS). Histopathological analysis suggests that l-theanine can suppress DSS-induced IBD with significant inhibition of inflammation in large and small intestinal tissues. Moreover, the 200 mg/kg/day l-theanine-treated DSS group had higher body and small intestine weights, a lower disease activity index and expression of inflammatory factors than the DSS group without pre-treatment. In RNA sequencing and tandem mass tag labeling analyses, large number of mRNAs and proteins expression level differed when compared with the DSS-induced rats with and without 200 mg/kg/day l-theanine pre-treatment. * Wen‑jun Xiao [email protected] 1
Key Lab of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, Hunan, People’s Republic of China
2
National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, Hunan, People’s Republic of China
3
CAS Key Laboratory of Agro‑ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, Hunan, People’s Republic of China
4
School of Pharmacy, Shaoyang University, Shaoyang 422000, Hunan, People’s Republic of China
5
School of Medicine, The University of Sydney, Sydney, NSW 2006, Australia
6
Forevergen Biosciences Center, Guangzhou 51000, Ghuangdong, People’s Republic of China
Moreover, Kyoto Encyclopedia of Genes and Genomes pathway analysis indicates the anti-inflammatory activities of l-theanine in DSS-induced IBD, with a high representation of genes in “Cholesterol metabolism” and “Retinol metabolism” pathways. Analysis of protein–protein interaction networks further indicates the involvement of these two pathways. These studies suggest that medium-dose l-theanine pre-treatment could ameliorate DSS-induced IBD through molecular mechanisms involving cholesterol and retinol metabolism. Keywords l-Theanine · Inflammatory bowel disease · Dextran sulfate sodium · Anti-inflammatory · Protective effects
Introduction Inflammatory bowel disease (IBD) encompasses a range of inflammatory gastrointestinal disorders (Hirten et al. 2018; Lee et al. 2018; Bernstein et al. 2019). The IBD entity includes three distinct diseases: Crohn’s Disease, ulcerative colitis and indeterminate colitis (
Data Loading...
