Protein Self-Organization Patterns in Dried Serum Reveal Changes in B-Cell Disorders
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ORIGINAL RESEARCH ARTICLE
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Protein Self-Organization Patterns in Dried Serum Reveal Changes in B-Cell Disorders Anthony A. Killeen,1 Natalya Ossina,2 Ronald C. McGlennen,1 Sharon Minnerath,1 John Borgos,3 Vadim Alexandrov4 and Armen Sarvazyan2 1 2 3 4
Department of Laboratory Medicine & Pathology, University of Minnesota, Minneapolis, Minnesota, USA Artann Laboratories, West Trenton, New Jersey, USA Cyphermetrics Inc, St Paul, Minnesota, USA PsychoGenics Inc., Tarrytown, New York, USA
Abstract
Background: Detection of serum monoclonal proteins is a common laboratory analysis used in the evaluation of patients with B-cell disorders. Since many individuals with elevated immunoglobulin have no symptoms, it is important to have simple methods for initial screening of patients with suspected B-cell disorders. Methods: Samples of serum from healthy donors and from patients with elevated immunoglobulin levels were tested using a technology named Droplet MicroChromatography (DMC). DMC was developed at Artann Laboratories (West Trenton, New Jersey, USA) for the rapid assessment of changes in the composition of serum. DMC is based on the dynamics of the sediment pattern formation during drying of a fluid microdroplet. Results: Results of this pilot study confirm the hypothesis that the pattern formation created by drying droplets of serum would differ between normal samples and those containing monoclonal proteins. Reproducible differences in the patterns formed by the two types of specimens are shown. Strong correlation between abnormally elevated levels of immunoglobulins in the serum of myeloma patients and the patterns formed by drying droplets of serum indicates that the DMC technique may be suitable for semi-quantitative analysis of serum samples. We also demonstrate that computer identification of the drying droplet structure and dynamics is a tractable issue. Conclusions: DMC has significant diagnostic potential and can serve as a basis for development of a simple, rapid, and inexpensive method for initial screening of patients suspected of having multiple myeloma and other pathologies of lymphoid origin that are associated with the overproduction of monoclonal immunoglobulins. The DMC test requires only ≈1μL of serum and could therefore be performed in any facility where it is safe to work with serum.
Background Detection of serum monoclonal proteins is a common laboratory analysis used in the evaluation of patients with B-cell disorders. There are several pathologies of lymphoid origin that are associated with abnormal overproduction of immunoglobulins; namely, multiple myeloma (MM),[1] Waldenstrom’s macroglobulinemia (WM),[2] monoclonal mammopathy of undetermined significance (MGUS),[3] and amyloidosis.[2] According to the Leukemia and Lymphoma Society,[4] an estimated 16 570 new cases of myeloma will be diagnosed in the US
in 2006. Primary amyloidosis accounts for ≈4000 deaths per year in the US. WM accounts for ≈2% of all hematologic malignancies. At pr
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