Protein-Silica Hybrid Submicron Particles: Preparation and Characterization
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ORIGINAL ARTICLE
Protein‑Silica Hybrid Submicron Particles: Preparation and Characterization Jaime Vega‑Chacón1,2 · Mohamad Tarhini1 · Noureddine Lebaz1 · Miguel Jafelicci Jr2 · Nadia Zine3 · Abdelhamid Errachid3 · Abdelhamid Elaissari1 Received: 24 January 2020 / Accepted: 15 April 2020 © The Tunisian Chemical Society and Springer Nature Switzerland AG 2020
Abstract Purpose Hybrid nanoparticles are an interesting type of particles currently employed in nanomedicine. They can combine the therapeutic and diagnostic functions of their components into one single nanosystem and therefore, can be tweaked according to their applications and desired results. The purpose of this work is to develop an easily tuned nano-system of bovine serum albumin-silica hybrid nanoparticles. Methods BSA nanoparticles were prepared by nanoprecipitation process using water as the solvent phase and ethanol as the non-solvent. Silica was produced through a sol–gel process and deposited inside the BSA nanoparticles via template deposition. The effect of initial concentrations of raw materials on the structure and colloidal properties of the system was evaluated using FTIR, TGA, TEM, and zeta potential measurements. Results A hybrid structure of BSA-silica was obtained. The results suggest that the particles size, isoelectric point and silica content of the hybrid nanoparticles can be easily tuned by controlling adequately the concentration of TEOS and ammonia. Conclusion Our method can be considered as a novel approach for hybrid nanoparticle synthesis. To our knowledge, we report for the first time the synthesis of silica-BSA hybrid particles with a non-core–shell structure. In addition, we proved that that the particles size, isoelectric point and silica content of the hybrid nanoparticles can be easily tuned by controlling adequately the concentration of TEOS and ammonia. Keywords BSA · Silica · Hybrid nanoparticles · Characterization
1 Introduction The preparation of nanoparticles is a research field which had a high technological development and commercial interest during the last decades due to the production of materials with unique properties that have been widely employed. Particularly, in the biomedical and pharmaceutical domains, the preparation of nanoparticles has received considerable attention owing to their capacity of interacting intimately * Mohamad Tarhini mohamad.tarhini@univ‑lyon1.fr 1
University Lyon, University Claude Bernard Lyon-1, CNRS, LAGEPP-UMR 5007, 43 Boulevard du 11 Novembre 1918, 69100 Villeurbanne, France
2
Institute of Chemistry, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil
3
Institute of Analytical Sciences, UMR 5280, Claude Bernard Lyon 1 University, University of Lyon, Villeurbanne, France
with biological structures [1]. Thereby, nanoparticles have been used as delivery systems (drugs, genes, proteins) [2–4], therapeutic agents (photothermal therapy, magnetic hyperthermia therapy, photodynamic therapy) [5–7] and imaging agents (magnetic resonance imaging, fluore
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