Pyramiding Ty - 1/Ty - 3, Ty - 2, ty - 5 and ty - 6 genes into tomato hybrid to develop resistance against tomato leaf c
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ORIGINAL ARTICLE
Pyramiding Ty-1/Ty-3, Ty-2, ty-5 and ty-6 genes into tomato hybrid to develop resistance against tomato leaf curl viruses and recurrent parent genome recovery by ddRAD sequencing method Pardhasaradhi Prabhandakavi1,2 • Rambabu Pogiri1 • Rakesh Kumar1 • Santanu Acharya1 • Ramchandran Esakky1 • M. Chakraborty1 • Rajasekhar Pinnamaneni3 • Sairam Reddy Palicherla1 Received: 19 December 2019 / Accepted: 8 November 2020 Ó Society for Plant Biochemistry and Biotechnology 2020
Abstract Tomato leaf curl virus (ToLCV) is a begomovirus transmitted by whitefly (Bemisia tabaci) and causes Tomato (yellow) leaf curl disease (TYLCD/ToLCD) in many parts of India and rest of the world resulting in huge economic losses. Many Ty genes identified either from wild and/or cultivated tomato, which confers various levels of resistance to ToLCV. The current study aimed at stacking four important resistant genes (Ty-1/Ty-3, Ty-2, ty-5, and ty-6) in parental lines of a commercial tomato hybrid JKTH811 with the aid of the marker-assisted backcross breeding program. As a donor source, two pre-breeding lines, CLN3125K (Ty-1/Ty-3 and Ty-2) and FLA456 (ty-5 and ty-6) was used. Crossed population screened by using tightly linked Ty genes specific markers for foreground selection and attempted the fastest recovery of recurrent parental genome through NGS based ddRAD sequencing approach. Ty genes introgressed parental lines as well as the resulting F1 hybrids were tested for their bio-efficacy against ToLCNDV (New Delhi var.) under greenhouse and field conditions. Disease severity of Ty genes pyramided female lines and F1 hybrids were found as significantly lower when compared to original parental lines and hybrid. This report demonstrates the efficiency of Ty genes pyramiding from different donors in a hybrid against ToLCV and recovery of the recurrent genome using the ddRAD-seq platform in the minimum generations possible. Keywords Gene pyramiding Marker-assisted backcross breeding ddRAD-seq Tomato leaf curl virus disease Abbreviations ddRAD-seq Double digested restriction site-associated DNA-sequencing ToLCD Tomato leaf curl virus disease ToLCNDV Tomato leaf curl Newdelhi virus ToLCV Tomato leaf curl virus
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13562-020-00633-1) contains supplementary material, which is available to authorized users. & Pardhasaradhi Prabhandakavi [email protected] 1
Division of Biotechnology, JK Agri Genetics Ltd, Hyderabad 500016, India
2
Jawaharlal Nehru Technological University, Hyderabad 500085, India
3
Department of Biotechnology, Sreenidhi Institute of Science and Technology, Ghatkesar, Hyderabad 501301, India
Introduction The Tomato (Solanum lycopersicum L.) is the second most important vegetable crop grown extensively in tropical and subtropical regions of the world. Although the production of the crop affected by many diseases, Tomato (yellow) leaf curl disease (TYLCD/ToLCD), caused by Tomato leaf curl virus (ToLCV) regarded
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