Reconstruction of the absorption spectrum of Synechocystis sp. PCC 6803 optical mutants from the in vivo signature of in
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ORIGINAL ARTICLE
Reconstruction of the absorption spectrum of Synechocystis sp. PCC 6803 optical mutants from the in vivo signature of individual pigments David Fuente1,2,3 · Dusan Lazar4 · Jose Vicente Oliver‑Villanueva1 · Javier F. Urchueguía1 Received: 10 September 2020 / Accepted: 13 November 2020 © Springer Nature B.V. 2020
Abstract In this work, we reconstructed the absorption spectrum of different Synechocystis sp. PCC 6803 optical strains by summing the computed signature of all pigments present in this organism. To do so, modifications to in vitro pigment spectra were first required: namely wavelength shift, curve smoothing, and the package effect calculation derived from high pigment densities were applied. As a result, we outlined a plausible shape for the in vivo absorption spectrum of each chromophore. These are flatter and slightly broader in physiological conditions yet the mean weight-specific absorption coefficient remains identical to the in vitro conditions. Moreover, we give an estimate of all pigment concentrations without applying spectrophotometric correlations, which are often prone to error. The computed cell spectrum reproduces in an accurate manner the experimental spectrum for all the studied wavelengths in the wild-type, Olive, and PAL strain. The gathered pigment concentrations are in agreement with reported values in literature. Moreover, different illumination set-ups were evaluated to calculate the mean absorption cross-section of each chromophore. Finally, a qualitative estimate of light-limited cellular growth at each wavelength is given. This investigation describes a novel way to approach the cell absorption spectrum and shows all its inherent potential for photosynthesis research. Keywords Absorption · Spectrum · Light · Pigment · Modeling · Synechocystis · Photosystem
Introduction Light spectrum influence in photosynthesis
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11120-020-00799-8) contains supplementary material, which is available to authorized users. * David Fuente [email protected] 1
Instituto de Aplicaciones de las Tecnologías de la Información y de las Comunicaciones Avanzadas, Universitat Politècnica de València, Valencia, Spain
2
Department of Biophysics, Faculty of Science, Palacký University, Olomouc, Czech Republic
3
Department of Adaptation Biotechnologies, Global Change Research Centre, Academy of Science of the Czech Republic, Drásov, Czech Republic
4
Department of Biophysics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, Czech Republic
Light is a principal factor regulating photosynthesis and influencing its global efficiency because light is the fundamental energy source for photosynthetic organisms and in many environments radiation is a limiting factor. The impact of light on the photosynthetic apparatus has been widely researched since the second half of last century and more recently in silico q
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