Renal microvascular oxygen tension during hyperoxia and acute hemodilution assessed by phosphorescence quenching and exc
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REPORTS OF ORIGINAL INVESTIGATIONS
Renal microvascular oxygen tension during hyperoxia and acute hemodilution assessed by phosphorescence quenching and excitation with blue and red light Tension d’oxyge`ne microvasculaire re´nal pendant l’hyperoxie et l’he´modilution aigue¨ e´value´e par de´sactivation de la phosphorescence et excitation avec de la lumie`re bleue et rouge Kyle Chin, BMSc . Melina P. Cazorla-Bak, BVSc . Elaine Liu, MD . Linda Nghiem, RVT, RLAT . Yanling Zhang, MD, PhD . Julie Yu, MD . David F. Wilson, PhD . Sergei A. Vinogradov, PhD . Richard E. Gilbert, MD, PhD . Kim A. Connelly, MD, PhD . Roger G. Evans, PhD . Andrew J. Baker, MD . C. David Mazer, MD . Gregory M. T. Hare, MD, PhD Received: 31 May 2020 / Revised: 5 August 2020 / Accepted: 14 August 2020 Ó Canadian Anesthesiologists’ Society 2020
Abstract Purpose The kidney plays a central physiologic role as an oxygen sensor. Nevertheless, the direct mechanism by which this occurs is incompletely understood. We measured renal microvascular partial pressure of oxygen (PkO2) to determine the impact of clinically relevant conditions that acutely change PkO2 including hyperoxia and hemodilution.
Kyle Chin and Melina P. Cazorla-Bak contributed equally to this manuscript. K. Chin, BMSc E. Liu, MD Department of Anesthesia, St. Michael’s Hospital, 30 Bond Street, Toronto, ON M5B 1W8, Canada M. P. Cazorla-Bak, BVSc Department of Anesthesia, St. Michael’s Hospital, 30 Bond Street, Toronto, ON M5B 1W8, Canada Department of Physiology, University of Toronto, Toronto, ON, Canada L. Nghiem, RVT, RLAT Y. Zhang, MD, PhD Keenan Research Centre for Biomedical Science in the Li Ka Shing Knowledge Institute, St. Michael’s Hospital, Toronto, ON, Canada J. Yu, MD Deaprtment of Anesthesia and Perioperative Medicine, Western University, London, ON, Canada
Methods We utilized two-wavelength excitation (red and blue spectrum) of the intravascular phosphorescent oxygen sensitive probe Oxyphor PdG4 to measure renal tissue PO2 in anesthetized rats (2% isoflurane, n = 6) under two conditions of altered arterial blood oxygen content (CaO2): 1) hyperoxia (fractional inspired oxygen 21%, 30%, and 50%) and 2) acute hemodilutional anemia (baseline, 25% and 50% acute hemodilution). The mean arterial blood pressure (MAP), rectal temperature, arterial blood gases (ABGs), and chemistry (radiometer) were measured under each condition. Blue and red light enabled measurement of D. F. Wilson, PhD S. A. Vinogradov, PhD Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA R. E. Gilbert, MD, PhD Keenan Research Centre for Biomedical Science in the Li Ka Shing Knowledge Institute, St. Michael’s Hospital, Toronto, ON, Canada Division of Endocrinology, Department of Medicine, St. Michael’s Hospital, University of Toronto, Toronto, ON, Canada K. A. Connelly, MD, PhD Department of Physiology, University of Toronto, Toronto, ON, Canada Keenan Research Centre for Biomedical Science in the Li Ka Shing Knowledge Institute, St. Michae
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